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Anti-Neurofascin Antibody

NFASC, NRCAML, NF
Cat #: AIP-025
Alternative Name NFASC, NRCAML, NF
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)REKKDVPLGPEDPK, corresponding to amino acid residues 1142-1155 of rat NFASC (Accession P97685). Intracellular, C-terminus.
Accession (Uniprot) Number P97685
Gene ID 116690
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2756657.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat hippocampus (lanes 3 and 6) lysates:
    Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat hippocampus (lanes 3 and 6) lysates:
    1-3. Anti-Neurofascin Antibody (#AIP-025), (1:800).
    4-6. Anti-Neurofascin Antibody, preincubated with Neurofascin Blocking Peptide (#BLP-IP025).
References
  1. Volkmer, H. et al. (1992) J. Cell Biol. 118, 149.
  2. Ratcliffe, C.F. et al. (2001) J. Cell Biol. 154, 427.
  3. Charles, P. et al. (2002) Cur. Biol. 12, 217.
  4. Koroll, M. et al. (2001) J. Biol. Chem. 276, 10646.
Scientific background

Neurofascin belongs to the L1 subgroup of the immunoglobulin superfamily of cell adhesion molecules and is an axon associated surface glycoprotein implicated in axonal growth and fasciculation.

Neurofascin is comprised of four structural elements: At the NH2 terminus, neurofascin contains six Ig-like motifs of the C2 subcategory followed by four fibronectin type III (FNIII)-related repeats. Between the FNIII-like repeats and the plasma membrane spanning region, neurofascin contains a 75-amino acid residues rich in proline, alanine and threonine which might be the target of extensive O-linked glycosylation. A transmembrane segment is followed by a 113-amino acid residues-long cytoplasmic domain1.

Neurofascin associates with sodium channel β1 (NaVβ1) units via interaction of its first Ig-like domain and second fibronectin type III–like domain with the extracellular Ig-like domain of β1. NaVβ1 subunits localize to nodes of Ranvier with neurofascin in sciatic nerve axons. NaVβ1 and neurofascin are associated as early as postnatal day 5, during the period that nodes of Ranvier are in formation2.

Unlike L1 and other subgroup members, neurofascin is subjected to extensive alternative splicing that is regulated during embryonic development of the brain. An oligodendroglial isoform of neurofascin, NF155, serves as a glial receptor for the paranodin/Caspr-contactin complex. The extracellular domain of NF155 binds to the complex at the cell surface3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-Neurofascin Antibody (#AIP-025) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize NFASC from human, mouse, and rat samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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