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Anti-Neuronal Pentraxin Receptor (NPTXR)(extracellular) Antibody

NPR, NPTXR

Cat #: ANR-193
Alternative Name NPR, NPTXR
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RGLQDAGPRRDTMAD, corresponding to amino acid residues 162 - 176 of mouse NPTXR (Accession Q99J85). Extracellular, C-terminus
Accession (Uniprot) Number Q99J85
Gene ID NPTXR
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – identical; human – 14 out of 15 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ip*, lci*
Western blot
  • Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
    Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
    1-2. Anti-Neuronal Pentraxin Receptor (NPTXR)(extracellular) Antibody (#ANR-193), (1:400).
    3-4. Anti-Neuronal Pentraxin Receptor (NPTXR)(extracellular)  Antibody, preincubated with Neuronal Pentraxin Receptor (NPTXR)(extracellular) Blocking Peptide (BLP-NR193).
  • Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):
    Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):
    1-2. Anti-Neuronal Pentraxin Receptor (NPTXR)(extracellular) Antibody (#ANR-193), (1:400).
    3-4. Anti-Neuronal Pentraxin Receptor (NPTXR)(extracellular)  Antibody, preincubated with Neuronal Pentraxin Receptor (NPTXR)(extracellular) Blocking Peptide (BLP-NR193).
Immunohistochemistry
  • Expression of Neuronal Pentraxin Receptor in rat hippocampus.
    Expression of Neuronal Pentraxin Receptor in rat hippocampus.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Neuronal Pentraxin Receptor (NPTXR)(extracellular) Antibody (#ANR-193), (1:1200), followed by donkey anti-rabbit-biotin and streptavidin-Cy3. A. Staining in the rat hippocampal CA3 region, showed NPTXR immunoreactivity (red) in neuronal profiles (arrows) in the pyramidal layer (P). B. Pre-incubation of the antibody with Neuronal Pentraxin Receptor (NPTXR)(extracellular) Blocking Peptide (BLP-NR193), suppressed staining. Cell nuclei are stained with DAPI (blue).
References
  1. Lee, S-J. et al (2017) J Neurosci, 32, 1067.
  2. Lim et al (2019), Clin Chem Lab Med., 57, 1875.
  3. Kanda, M. et al (2020), Molec. Cancer, 19, 131.
Scientific background

Neuronal Pentraxin Receptor (NPTXR) belongs to the Pentraxin superfamily of proteins (NP) that is characterized by a pentraxin domain at the C-terminal end. It is the only pentraxin family member anchored to the cell membrane by a putative N-terminal transmembrane domain, as opposed to the other two family members, Neuronal Pentraxin1 (NP1) and Neuronal Pentraxin 2 (NP2) that are secreted proteins.  NPs can form homo pentamers and hetero pentamers via their pentraxin domains 1.

NPTXR was first identified as the receptor for the snake venom toxin taipoxin and was later found to bind to AMPARs at excitatory synapses and play important, roles in the activity-dependent regulation of neural circuits via this binding activity 1.

Lately, the levels of NPTXR protein in cerebrospinal fluid has been identified as a novel potential biomarker of Alzheimer’s disease progression and could have important utility in assessing treatment success in clinical trials 2.  In addition, NPTXR was identified as a molecule required for gastric cancer metastasis 3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/08/2022

Anti-Neuronal Pentraxin Receptor (NPTXR)(extracellular) Antibody (#ANR-193) is a highly specific antibody directed against an extracellular epitope of the mouse NPTXR. The antibody can be used in western blot and immunohistochemistry. It has been designed to recognize NPTXR from mouse, rat and human samples.

For research purposes only, not for human use
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