Anti-NPY1R Antibody

Neuropeptide Y1 receptor, Neuropeptide Y receptor type 1, NPYR, NPYY1
    Cat #: ANR-021
    Alternative Name Neuropeptide Y1 receptor, Neuropeptide Y receptor type 1, NPYR, NPYY1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, r
    May also work in: m*
    Immunogen
    • Peptide (C)RLKRRNNMMDKMRDNK, corresponding to amino acid residues 237-252 of human NPY1R (Accession P25929). 3rd intracellular loop.
    • Anti-NPY1R Antibody
    Accession (Uniprot) Number P25929
    Gene ID 4886
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - 14/16 amino acid residues identical.
    RRID AB_2040030.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.75 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
    Western blot
    • Anti-NPY1R Antibody
      Western blot analysis of Jurkat (lanes 1 and 4), K562 (lanes 2 and 5), and RBL (lanes 3 and 6) cell lysates and rat brain lysates (lanes 7 and 8):
      1,2,3,7. Anti-NPY1R Antibody (#ANR-021), (1:200).
      4,5,6,8. Anti-NPY1R Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-NPY1R Antibody
      Expression of neuropeptide Y1 receptor in rat striatum
      Immunohistochemical staining of rat striatum using Anti-NPY1R Antibody (#ANR-021). A. NPY1R (red) appears in the striatal matrix (white asterisk) and in medium-size cells in the matrix (arrows). B. Parvalbumin (green) appears in the striatal matrix. C. Confocal merge of NPY1R and parvalbumin indicates that NPY1R is restricted to granule cells. DAPI is used as the counterstain (blue).
    Immunocytochemistry
    • Anti-NPY1R Antibody
      Expression of neuropeptide Y1 receptor in DRG neurons
      Immunocytochemical staining of a primary culture of rat dorsal root ganglion (DRG) neurons. A, D. A paraformaldehyde-fixed and permeabilized DRG primary culture was stained with Anti-NPY1R Antibody (#ANR-021), (1:100), followed by Alexa-555-conjugated goat-anti-rabbit secondary antibody. B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of A and B. F. Merged images of D and E.
      Magnification:
      A-C: x20
      E-F: x100
    References
    1. Stanic, D. et al. (2006) J. Comp. Neurol. 499, 357.
    2. Körner, M. et al. (2004) Clin. Cancer Res. 10, 8426.
    3. Sun, L. and Miller, R.J. (1999) J. Neurophysiol. 81, 1391.
    4. Bournat, J.C. and Allen, J.M. (2001) Brain Res. Mol. Brain Res. 90, 149.
    5. Wheway, J. et al. (2005) J. Exp. Med. 202, 1527.
    Scientific background

    Neuropeptide Y (NPY) is a 36-amino acid peptide neurotransmitter in the central (CNS) and peripheral nervous systems.1 It belongs to the NPY family, together with the peptide YY (PYY) and the pancreatic polypeptide (PP). NPY is involved in regulation of a broad range of homeostatic functions such as eating behavior, hypertension, and reproduction.2

    NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R) and Y6 (NPY6R).2 Four of these, Y1, Y2, Y4, and Y5, are expressed in humans.

    The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.

    Activation of these receptors by NPY produces effects on [Ca2+]i, adenylate cyclase, and a number of ion channels.Based on the high frequency and density of NPY receptors in steroid hormone-producing tumors, the NPY receptors might be of potential use in tumor management.2

    NPY1R is considered to be a postsynaptic receptor. In the CNS, NPY1Rs are concentrated in the cortical areas of the brain, cerebral cortex, olfactory nucleus, hippocampus, hypothalamic nuclei, and thalamic nuclei.4 In addition, this receptor has a bimodal role in the immune system, serving as a strong negative regulator on T cells as well as a key activator of antigen-presenting cell function.5

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 17/02/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the human neuopeptide Y1 receptor (NPY1R). Anti-NPY1R Antibody (#ANR-021) can be used in western blot analysis, immunohistochemistry, and immunocytochemical applications. It has been designed to recognize NPY1R from human, rat, and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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