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Anti-NPY5R Antibody

Neuropeptide Y5 receptor, Neuropeptide Y receptor type 5, NPY Y5 receptor, NPYY5

Cat #: ANR-025
Alternative Name Neuropeptide Y5 receptor, Neuropeptide Y receptor type 5, NPY Y5 receptor, NPYY5
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide SFIRKHRRRYSKKTAC, corresponding to amino acid residues 291-306 of rat NPY5R (Accession Q63634). 3rd intracellular loop.
Accession (Uniprot) Number Q63634
Gene ID 25340
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, bovine, canis - identical; human - 15/16 amino acid residues identical.
RRID AB_2040036.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.7 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat brain membrane:
    Western blot analysis of rat brain membrane:
    1. Anti-NPY5R Antibody (#ANR-025), (1:200).
    2. Anti-NPY5R Antibody, preincubated with NPY5R Blocking Peptide (#BLP-NR025).
Immunohistochemistry
  • Expression of neuropeptide Y5 receptor in rat striatum
    Expression of neuropeptide Y5 receptor in rat striatum
    Immunohistochemical staining of rat striatum using Anti-NPY5R Antibody (#ANR-025). A. NPY5R (red) appears in the striatal matrix (asterisk) and in nerve cells in the matrix (vertical arrows). B. Parvalbumin (green) appears in the striatal matrix. C. Merge of NPY5R and parvalbumin demonstrates co-localization in a sub-set of cells (horizontal arrows). DAPI is used as the counterstain (blue).
Immunocytochemistry
  • Expression of neuropeptide Y5 receptor in rat DRG
    Expression of neuropeptide Y5 receptor in rat DRG
    Immunocytochemical staining of a primary culture of rat dorsal root ganglion (DRG) neurons. A, D. A paraformaldehyde-fixed and permeabilized DRG primary culture was stained with Anti-NPY5R Antibody (#ANR-025), (1:100) followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody. B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B. F. Merged images of panels D and E.
    Magnification:
    A-C: x20
    E-F: x100
References
  1. Stanic, D. et al. (2006) J. Comp. Neurol. 499, 357.
  2. Körner, M. et al. (2004) Clin. Cancer Res. 10, 8426.
  3. Sun, L. and Miller, R.J. (1999) J. Neurophysiol. 81, 1391.
  4. Kamiji, M.M. et al. (2007) Endocr. Rev. 28, 664.
Scientific background

Neuropeptide Y (NPY) is a 36 amino acid peptide neurotransmitter present in the central and peripheral nervous systems.1 NPY is involved in the regulation of a broad range of functions such as eating behavior, hypertension, and reproduction.2

NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R).2 Four of these (Y1, Y2, Y4, and Y5) are expressed in humans.2 The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.

Activation of these receptors by NPY affects [Ca2+]i, adenylate cyclase signalling, and a number of ion channel functions.Based on their high frequency and density in steroid hormone-producing tumors, the NPYRs might be of potential use in tumor management.2

NPY5R as well as NPY1R and NPY2R are expressed in the hypothalamus, in the brain stem, and in peripheral tissues, such as blood vessels, lung, kidney, adrenal glands, stomach, colon, heart, pancreas, and intestine. NPY5R has been postulated as the mediator of the feeding response to NPY and related peptides (125 in Kamiji, M. M). Its localization in the brain is consistent with this hypothesis.4

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat neuropeptide Y5 receptor (NPY5R). Anti-NPY5R Antibody (#ANR-025) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize NPY5R from rat, human and mouse samples.

For research purposes only, not for human use

Applications

Citations

Citations
Immunohistochemistry citations
  1. Rat retinal sections (1:2000).
    Martins, J. et al. (2015) ASN. Neuro. 7, 4.
Immunocytochemistry citations
  1. Rat retinal ganglion cells (RGC), (1:250).
    Martins, J. et al. (2015) ASN. Neuro. 7, 4.

Specifications

Scientific Background

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