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Anti-Neurotensin Receptor 1 (extracellular) Antibody

NTR1, NTSR1, NTS1, High-affinity levocabastine-insensitive neurotensin receptor, NTRH, NTRR
Cat #: ANT-015
Alternative Name NTR1, NTSR1, NTS1, High-affinity levocabastine-insensitive neurotensin receptor, NTRH, NTRR
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, r
May also work in: m*
Immunogen
  • Peptide EQNRSADGQHAGGLVC corresponding to amino acid residues 209-224 of human NTSR1 (Accession P30989). 2nd extracellular loop.
Accession (Uniprot) Number P30989
Gene ID 4923
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 14/16 amino acid residues identical; rat - 12/16 amino acid residues identical.
RRID AB_2040038.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ih, lci, wb
May also work in: ip*
Western blot
  • Western blot analysis of human colon cancer HT-29 (lanes 1 and 4), human lung small cell carcinoma NCI-H526 (lanes 2 and 5) and human breast adenocarcinoma MDA-MB-468 (lanes 3 and 6) cell lysates:
    Western blot analysis of human colon cancer HT-29 (lanes 1 and 4), human lung small cell carcinoma NCI-H526 (lanes 2 and 5) and human breast adenocarcinoma MDA-MB-468 (lanes 3 and 6) cell lysates:
    1-3. Anti-Neurotensin Receptor 1 (extracellular) Antibody (#ANT-015), (1:200).
    4-6. Anti-Neurotensin Receptor 1 (extracellular) Antibody, preincubated with Neurotensin Receptor 1 (extracellular) Blocking Peptide (#BLP-NT015).
Immunohistochemistry
  • Expression of NTSR1 in human breast cancer
    Expression of NTSR1 in human breast cancer
    Immunohistochemical staining of human breast cancer cells using Anti-Neurotensin Receptor 1 (extracellular) Antibody (#ANT-015), (1:100). Staining (brown color) is specific for epithelium-derivated malignant cells. Hematoxilin is used as the counterstain.
  • Expression of NTSR1 in rat brain
    Expression of NTSR1 in rat brain
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Neurotensin Receptor 1 (extracellular) Antibody (#ANT-015), (1:100). A. NTSR1 staining (green) appears in cells of the diagonal band region. B. The same section was stained with Choline acetyltransferase (ChAT) (red), a marker of cholinergic neurons. C. Merge of the two images demonstrates expression of NTSR1 in cholinergic neurons. Arrows point at examples of colocalization. Cell nuclei were stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of NTSR1 in live intact human promyelocytic leukemia HL-60 cell line:
    Cell surface detection of NTSR1 in live intact human promyelocytic leukemia HL-60 cell line:
    ___ Cells + FITC-conjugated goat anti rabbit antibody.
    ___ Cells + Anti-Neurotensin Receptor 1 (extracellular) Antibody (#ANT-015), (1:20) + FITC-conjugated goat-anti-rabbit antibody.
  • The control antigen is not suitable for this application.
Live cell imaging / Immunocytochemistry
  • Expression of NTSR1 in human MCF-7 cells
    Expression of NTSR1 in human MCF-7 cells
    Cell surface detection of NTSR1 in live intact human MCF-7 breast adenocarcinoma cell line. A. Extracellular staining of cells with Anti-Neurotensin Receptor 1 (extracellular) Antibody (#ANT-015), (1:20), followed by Alexa 488-conjugated goat anti-rabbit antibody (green staining). B. Live view of the cells. C. Merge of A and B.
References
  1. Vincent, J.P. et al. (1999) Trends Pharmacol. Sci. 20, 302.
  2. Binder, E.B. et al. (2001) Pharmacol. Rev. 53, 453.
  3. Souaze, F. et al. (2006) Cancer Res. 66, 6243.
Scientific background

Neurotensin receptor 1 (NTR1) is one of three receptors that mediate the effects of the tridecapeptide neurotensin. Neurotensin is synthesized and secreted from neurons in the central neural system (CNS) and from endocrine cells in the gastrointestinal tract.

NTR1 and NTR2 belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily while the third neurotensin receptor NTR3 (also called Sortilin) is a type I membrane protein with a large extracellular domain. Both NTR1 and NTR3 bind neurotensin with high affinity while NTR2 binds it with low affinity.1

NTR1 signals preferentially through Gaq, resulting in the activation phopholipase C and intracellular Ca2+ mobilization, although coupling with Gi/o and to Gs has been observed in some expression systems.

NTR1 is mainly expressed in the brain and gastrointestinal tract. In the brain, NTR1 mediates body temperature regulation, feeding behavior and locomotion regulation. One of the best studied roles of NTR1 is the regulation of dopamine release suggesting that NTR1 can be a suitable target for the development of a new class of anti-psychotics.2  

In the periphery, NTR1 expression has been linked to the control of intestine motility and in the stimulation of growth of the intestine mucosa in both physiological and pathological conditions. NTR1 has been shown to be upregulated in several human cancers where it stimulates tumor growth in response to locally produced neurotensin peptide.3

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Anti-Neurotensin Receptor 1 (extracellular) Antibody (#ANT-015) is a highly specific antibody directed against an epitope of the human NTSR1. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and flow cytometry applications. It has been designed to recognize NTSR1 from human, mouse, and rat samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Immunocytochemistry citations
  1. Human B cells (1:200).
    Saada, S. et al. (2012) J. Immunol. 189, 5293.
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