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Anti-Neurotensin Receptor 2 Antibody

NTR2, NTSR2, Levocabastine-sensitive neurotensin receptor
Cat #: ANT-016
Alternative Name NTR2, NTSR2, Levocabastine-sensitive neurotensin receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide CGEQHSLVPLPQEAPE, corresponding to amino acid residues 377-392 of rat NTSR2 (Accession Q63384). Intracellular, C-terminus.
Accession (Uniprot) Number Q63384
Gene ID 64636
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 14/16 amino acid residues identical.
RRID AB_2040040.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity Unlikely to recognize human samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat dorsal root ganglion lysates:
    Western blot analysis of rat dorsal root ganglion lysates:
    1. Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:200).
    2. Anti-Neurotensin Receptor 2 Antibody, preincubated with Neurotensin Receptor 2 Blocking Peptide (#BLP-NT016).
  • Western blot analysis of rat brain lysates:
    Western blot analysis of rat brain lysates:
    1. Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:200).
    2. Anti-Neurotensin Receptor 2 Antibody, preincubated with Neurotensin Receptor 2 Blocking Peptide (#BLP-NT016).
  • Western blot analysis of mouse brain lysates:
    Western blot analysis of mouse brain lysates:
    1. Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:400).
    2. Anti-Neurotensin Receptor 2 Antibody, preincubated with Neurotensin Receptor 2 Blocking Peptide (#BLP-NT016).
Immunohistochemistry
  • Expression of NTR2 in rat mediobasal hypothalamus
    Expression of NTR2 in rat mediobasal hypothalamus
    Immunohistochemical staining of perfusion-fixed brain frozen rat mediobasal (MB) hypothalamus sections using Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:100), (green). A. NTR2 appears in neuronal fibers marked by white arrows. B. In the same section, there is staining for glial fibrillary acidic protein (red fluorescence), a marker of astrocytes. C. Merging the two images demonstrates that NTR2 is not localized in astrocytes. DAPI is used as the counterstain (blue).
  • Expression of NTR2 in rat stomach
    Expression of NTR2 in rat stomach
    Immunohistochemical staining of paraffin-embedded rat stomach sections using Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:50), (brown). Staining is specific for parietal cells (arrows) of the gastric glands. Hematoxilin is used as the counterstain.
Immunocytochemistry
  • Expression of NTR2 in rat C6 glioma cells
    Expression of NTR2 in rat C6 glioma cells
    Immunocytochemical staining of rat C6 glioma cells. A. Paraformaldehyde fixed and permeabilized rat C6 glioma cells were stained with Anti-Neurotensin Receptor 2 Antibody (#ANT-016), (1:200), followed by Alexa-555 conjugated goat-anti-rabbit- secondary antibody (orange staining). Nuclei were visualized with the cell permeable dye Hoechst 33342 (blue staining). B. Live view of the same field as in (A).
References
  1. Vincent, J.P. et al. (1999) Trends Pharmacol. Sci. 20, 302.
  2. Schulz, S. et al. (2006) J. Endocrinol. 191, 121.
  3. Maeno, H. et al. (2004) Brain Res. 998, 122.
Scientific background

Neurotensin receptor 2 (NTR2) is one of three receptors that mediate the effects of the tridecapeptide neurotensin. Neurotensin is synthesized and secreted from neurons in the central neural system (CNS) and from endocrine cells in the gastrointestinal tract.

NTR2 and NTR1 belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily while the third neurotensin receptor NTR3 (also called Sortilin) is a type I membrane protein with a large extracellular domain. Both NTR1 and NTR3 bind neurotensin with high affinity while NTR2 binds it with low affinity.1

NTR2 signals preferentially through Gaq, resulting in the activation phopholipase C and intracellular Ca2+mobilization. However, the exact signaling mechanisms subsequent to NTR2 activation appear to depend on the species and on the cellular system in which the receptor is studied.

NTR2 is expressed mainly in the central nervous system and dorsal root ganglion (DRG) neurons but has been also observed in peripheral tissues such as gastric parietal cells where it probably mediates the neurotensin-induced effects on gastric acid secretion.2

The most established physiological role of NTR2 is its role in pain transmission as NTR2-defficient mice showed that NTR2 is the likely mediator of the neurotensin-induced analgesic responses.3

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope at the intracellular C-terminal domain of the rat Neurotensin receptor type 2. Anti-Neurotensin Receptor 2 Antibody (#ANT-016) can be used in western blot, immunohistochemical and immunocytochemical applications, and recognizes NTR2 from rat and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
More product citations
  1. Saada, S. et al. (2012) J. Immunol. 189, 5293.
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