- Peptide (C)IQKENSREALVEPA, corresponding to residues 261-274 of rat NHERF-1 (Accession Q9JJ19). Between the PDZ2 and the ERM binding domains.
- Reczek, D. et al. (1997) J. Cell Biol. 139, 169.
- Voltz, J.W. et al. (2001) Oncogene 20, 6309.
- Stemmer-Rachamimov, A.O. et al. (2001) Am. J. Pathol. 158, 57.
The Na+/H+ exchange regulatory factor (EBP-50 or NHERF-1) is a 55 kD cytoplasmic protein adaptor that recruits a wide variety of cellular proteins.
Many of the interacting proteins do so through the two tandem PDZ domains (protein-binding domains conserved in the mammalian synaptic protein PSD-95/DlgA/ZO-1) and the C-terminal ERM (ezrin, radixin, moesin) binding region.
NHERF-1 was first identified as an adaptor necessary for the function of the Na+/H+ exchanger isoform 3 (NHE3) in renal apical cells.1 Since then it has been identified in cells of epithelial origin in several tissues such as gastrointestinal and lung.
NHERF-1 has been shown to interact with a growing number of proteins including ion channels (ROMK, CFTR, P2Y1, TRPC4 and TRPC6), growth factor receptors (PDGFR), phospholipase C isoforms (PLCb1, PLCb2, PLCb3), non-receptor protein tyrosine kinases (YAP65) and several cytoskeletal proteins that link membrane proteins to the underlying actin cytoskeleton.2
Recently, it has been shown that NHERF-1 expression was elevated in breast tumors compared to the expression in adjacent normal tissue.3
Application key:
Species reactivity key:
Anti-NHERF-1/EBP50 Antibody (#APZ-006) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize NHERF-1 from rat, mouse, and human samples.
Applications
Specifications
Scientific Background
Citations
- Laufer, J. et al. (2009) Cell. Physiol. Biochem. 23, 25.
- Böhmer, C. et al. (2008) Cell. Physiol. Biochem. 22, 705.
- Böhmer, C. et al. (2007) FEBS Lett. 581, 5586.
- Tan, P.C. et al. (2006) Stem Cells 24, 1150.
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