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Anti-NHERF-1/EBP50 Antibody

Na+/H+ exchange regulatory cofactor 1, Ezrin-radixin-moesin-binding phosphoprotein 50, SLC9A3R1

Cat #: APZ-006
Alternative Name Na+/H+ exchange regulatory cofactor 1, Ezrin-radixin-moesin-binding phosphoprotein 50, SLC9A3R1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: r
Immunogen
  • Peptide (C)IQKENSREALVEPA, corresponding to residues 261-274 of rat NHERF-1 (Accession Q9JJ19). Between the PDZ2 and the ERM binding domains.
Accession (Uniprot) Number Q9JJ19
Gene ID 59114
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical.
RRID AB_2039834.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.2 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
Western blot
  • Western blotting of rat kidney membranes:
    Western blotting of rat kidney membranes:
    1. Anti-NHERF-1/EBP50 Antibody (APZ-006), (1:200).   
    2. Anti-NHERF-1/EBP50 Antibody, preincubated with NHERF-1/EBP50 Blocking Peptide (#BLP-PZ006).
References
  1. Reczek, D. et al. (1997) J. Cell Biol. 139, 169.
  2. Voltz, J.W. et al. (2001) Oncogene 20, 6309.
  3. Stemmer-Rachamimov, A.O. et al. (2001) Am. J. Pathol. 158, 57.
Scientific background

The Na+/H+ exchange regulatory factor (EBP-50 or NHERF-1) is a 55 kD cytoplasmic protein adaptor that recruits a wide variety of cellular proteins.

Many of the interacting proteins do so through the two tandem PDZ domains (protein-binding domains conserved in the mammalian synaptic protein PSD-95/DlgA/ZO-1) and the C-terminal ERM (ezrin, radixin, moesin) binding region.

NHERF-1 was first identified as an adaptor necessary for the function of the Na+/Hexchanger isoform 3 (NHE3) in renal apical cells.1 Since then it has been identified in cells of epithelial origin in several tissues such as gastrointestinal and lung.

NHERF-1 has been shown to interact with a growing number of proteins including ion channels (ROMK, CFTR, P2Y1, TRPC4 and TRPC6), growth factor receptors (PDGFR), phospholipase C isoforms (PLCb1, PLCb2, PLCb3), non-receptor protein tyrosine kinases (YAP65) and several cytoskeletal proteins that link membrane proteins to the underlying actin cytoskeleton.2

Recently, it has been shown that NHERF-1 expression was elevated in breast tumors compared to the expression in adjacent normal tissue.3

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Anti-NHERF-1/EBP50 Antibody (#APZ-006) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize NHERF-1 from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
More product citations
  1. Laufer, J. et al. (2009) Cell. Physiol. Biochem. 23, 25.
  2. Böhmer, C. et al. (2008) Cell. Physiol. Biochem. 22, 705.
  3. Böhmer, C. et al. (2007) FEBS Lett. 581, 5586.
  4. Tan, P.C. et al. (2006) Stem Cells 24, 1150.
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