Name: Anti-NHERF-1/EBP50 Antibody
Brand: Alomone Labs
SKU: APZ-006

Overview

Cat #: APZ-006

Alternative Name Na⁺/H⁺ exchange regulatory cofactor 1, Ezrin-radixin-moesin-binding phosphoprotein 50, SLC9A3R1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: r

Immunogen

Peptide (C)IQKENSREALVEPA, corresponding to residues 261-274 of rat NHERF-1 (Accession Q9JJ19). Between the PDZ2 and the ERM binding domains.

Accession (Uniprot) Number Q9JJ19

Gene ID 59114

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical.

RRID AB_2039834.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN₃.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 0.2 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: wb

Western blot

Western blotting of rat kidney membranes:
1. Anti-NHERF-1/EBP50 Antibody (APZ-006), (1:200).
2. Anti-NHERF-1/EBP50 Antibody, preincubated with NHERF-1/EBP50 Blocking Peptide (#BLP-PZ006).

References

Reczek, D. et al. (1997) J. Cell Biol. 139, 169.
Voltz, J.W. et al. (2001) Oncogene 20, 6309.
Stemmer-Rachamimov, A.O. et al. (2001) Am. J. Pathol. 158, 57.

Scientific background

The Na⁺/H⁺ exchange regulatory factor (EBP-50 or NHERF-1) is a 55 kD cytoplasmic protein adaptor that recruits a wide variety of cellular proteins.

Many of the interacting proteins do so through the two tandem PDZ domains (protein-binding domains conserved in the mammalian synaptic protein PSD-95/DlgA/ZO-1) and the C-terminal ERM (ezrin, radixin, moesin) binding region.

NHERF-1 was first identified as an adaptor necessary for the function of the Na⁺/H⁺exchanger isoform 3 (NHE3) in renal apical cells.¹ Since then it has been identified in cells of epithelial origin in several tissues such as gastrointestinal and lung.

NHERF-1 has been shown to interact with a growing number of proteins including ion channels (ROMK, CFTR, P2Y1, TRPC4 and TRPC6), growth factor receptors (PDGFR), phospholipase C isoforms (PLCb1, PLCb2, PLCb3), non-receptor protein tyrosine kinases (YAP65) and several cytoskeletal proteins that link membrane proteins to the underlying actin cytoskeleton.²

Recently, it has been shown that NHERF-1 expression was elevated in breast tumors compared to the expression in adjacent normal tissue.³

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-NHERF-1/EBP50 Antibody (#APZ-006) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize NHERF-1 from rat, mouse, and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 10/04/2023

Applications

Citations

More product citations

Laufer, J. et al. (2009) Cell. Physiol. Biochem. 23, 25.
Böhmer, C. et al. (2008) Cell. Physiol. Biochem. 22, 705.
Böhmer, C. et al. (2007) FEBS Lett. 581, 5586.
Tan, P.C. et al. (2006) Stem Cells 24, 1150.

Specifications

Scientific Background

Specific Control Product

NHERF-1/EBP50 Blocking Peptide (#BLP-PZ006) is the original antigen used for immunization during Anti-NHERF-1/EBP50 Antibody (#APZ-006) generation. The blocking peptide binds and ‘blocks’ Anti-NHERF-1/EBP50 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

NHERF-1/EBP50 Blocking Peptide (#BLP-PZ006)

Shipping and Ordering Information

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Anti-NHERF-1/EBP50 Antibody