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Anti-Nicotinic Acetylcholine Receptor α5 (CHRNA5) (extracellular) Antibody

nAChR α5, Cholinergic receptor nicotinic alpha 5, NACHRA5, Neuronal acetylcholine receptor subunit alpha-5

Cat #: ANC-005
Alternative Name nAChR α5, Cholinergic receptor nicotinic alpha 5, NACHRA5, Neuronal acetylcholine receptor subunit alpha-5
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)EGASTKTVVRYNGT, corresponding to amino acid residues 129-142 of rat nAChRα5 (Accession P20420). Extracellular, N-terminus.
Accession (Uniprot) Number P20420
Gene ID P20420
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical; human - 12/14 amino acid residues identical.
RRID AB_2756674.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) enriched brain membranes:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) enriched brain membranes:
    1,2. Anti-Nicotinic Acetylcholine Receptor α5 (CHRNA5) (extracellular) Antibody (#ANC-005), (1:200).
    3,4. Anti-Nicotinic Acetylcholine Receptor α5 (CHRNA5) (extracellular) Antibody, preincubated with Nicotinic Acetylcholine Receptor α5/CHRNA5 (extracellular) Blocking Peptide (#BLP-NC005).
References
  1. Jensen, A.A. et al. (2005) J. Med. Chem. 48, 4705.
  2. Millar, N.S. and Harkness, P.C. (2008) Mol. Membr. Biol. 25, 279.
  3. Hogg, R.C. et al. (2003) Rev. Physiol. Biochem. Pharmacol. 147, 1.
  4. Fu, X.W. et al. (2009) Am. J. Respir. Mol. Biol. 41, 93.
  5. De Rosa, M.J. et al. (2009) Life Sci. 85, 449.
  6. Eggert, M. et al. (2013) PLoS ONE 8, e66157.
Scientific background

Nicotinic acetylcholine receptors (nAChRs) mediate the physiological effects of exogenous nicotine. They also play critical physiological roles throughout the brain and body by mediating cholinergic excitatory neurotransmission, modulating the release of neurotransmitters, and having longer-term effects on gene expression and cellular connections1.

nAChRs are pentameric complexes made up of combinations of a number of different nAChR subunits, which can be classified as α subunits and non-α subunits (‘structural’ subunits), which can be defined as β subunits when they are expressed in the vertebrate nervous system2. There are nine α subunits (α2-α10) and three β subunits (β2, β3, and β4) in the CNS3. Nicotinic receptors are assembled as combinations of α (2-6) and β (2-4) subunits. 

All α subunits are expressed in neuronal cells except for the α1 subunit which is specifically expressed in skeletal muscle. They are also expressed in non-neuronal cells such as bronchial epithelial cells4, as well lymphocytes5. In the CNS, the α5 subunit is widely expressed in the CA1 area of the hippocampus which affects learning, memory, attention, anxiety, and potentially drug seeking behaviors.

Nicotinic acetylcholine receptor α5 is associated with different aspects of smoking behavior as well as with smoking related disorders. In addition, it has been found to be upregulated in smokers or differentially expressed in lung tumor cells6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/06/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat nAChRα5. Anti-Nicotinic Acetylcholine Receptor α5 (CHRNA5) (extracellular) Antibody (#ANC-005) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize nAChRα5 from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Immunohistochemistry citations
  1. Mouse pancreas sections (1:200).
    Ganic, E. et al. (2016) Cell Rep. 14, 1991.
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