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Anti-NrCAM (extracellular) Antibody

Neuronal cell adhesion molecule, Nr-CAM, NgCAM-related cell adhesion molecule, Ankyrin-binding cell adhesion molecule, Neuronal surface protein Bravo, HBravo

Cat #: ANR-045
Alternative Name Neuronal cell adhesion molecule, Nr-CAM, NgCAM-related cell adhesion molecule, Ankyrin-binding cell adhesion molecule, Neuronal surface protein Bravo, HBravo
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KDNGELPN DERFSVD, corresponding to amino acid residues 583-597 of rat NrCAM (Accession P97686). Extracellular, N-terminus.
Accession (Uniprot) Number P97686
Gene ID 497815
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – 15/16 amino acid residues identical; human – 13/16 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), rat cerebellum (lanes 3 and 7) and human SH-SY5Y neuroblastoma cell (lanes 4 and 8) lysates:
    Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), rat cerebellum (lanes 3 and 7) and human SH-SY5Y neuroblastoma cell (lanes 4 and 8) lysates:
    1-4. Anti-NrCAM (extracellular) Antibody (#ANR-045), (1:200).
    5-8. Anti-NrCAM (extracellular) Antibody, preincubated with NrCAM (extracellular) Blocking Peptide (#BLP-NR045).
Immunohistochemistry
  • Expression of NrCAM in rat hippocampus.
    Expression of NrCAM in rat hippocampus.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-NrCAM (extracellular) Antibody (#ANR-045), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal dentate gyrus region, showed immunoreactivity (green) in neurons of the hilus (H, arrows). B. Pre-incubation of the antibody with NrCAM (extracellular) Blocking Peptide (BLP-NR045), suppressed staining. Cell nuclei are stained with DAPI (blue). G = granule layer.
Indirect flow cytometry
  • Cell surface detection of NrCAM by indirect flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells: 
    Cell surface detection of NrCAM by indirect flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells: 
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC 
    ___ Cells + Anti-NrCAM (extracellular) Antibody(#ANR-045), (2.5μg) + goat-anti-rabbit-FITC
References
  1. Mohan, V. et al. (2019) Front. Cell Dev. Biol. 7, 9.
  2. Brummer, T. et al. (2019) EMBO Mol. Med. 11, 4.
  3. Demyanenko, G.P. et al. (2014) J. Neurosci. 34, 11274.
Scientific background

NrCAM (Neuron-glia related cell adhesion molecule) is a cell adhesion molecule that belongs to the immunoglobulin-class (Ig) of the L1 family. NrCAM promotes axon growth and repulsion through trans homophilic binding of extracellular domains and intracellular coupling of the conserved cytoplasmic domains to the actin cytoskeleton. This interaction occurs through ankyrin B and ezrin-radixin-moesin adaptors. In addition, NrCAM is responsible for controlling dendritic spine densities, axonal guidance and targeting, and neurite outgrowth by acting as a co‐receptor molecule at the neuronal cell surface1,2.

NrCAM is highly expressed in the cortex1,3. Its structure contains a PDZ binding motif with the potential to bind PSD scaffold proteins PSD-95 and SAP102. The Ig domains of the protein mediate homophilic and heterophilic binding to multiple ligands2,3.

Genetic polymorphisms in the NrCAM gene are linked to autism spectrum disorders, intellectual disability and addictive behavior. NrCAM knockout mice demonstrate autism spectrum disorder related behaviors including impaired sociability, cognitive inflexibility, hypersensitivity to sensory stimuli and altered visual cortical responses.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-NrCAM (extracellular) Antibody (#ANR-045) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize NrCAM from human, rat, and mouse samples.

For research purposes only, not for human use
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