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Anti-NRG2 (extracellular) Antibody

Neuregulin-2, Pro-neuregulin-2 membrane-bound isoform, Pro-NRG2, Neural- and thymus-derived activator for ErbB kinases, NTAK

Cat #: ANR-114
Alternative Name Neuregulin-2, Pro-neuregulin-2 membrane-bound isoform, Pro-NRG2, Neural- and thymus-derived activator for ErbB kinases, NTAK
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)RDIRIKYGNGRKNSR, corresponding to amino acid residues 297-311 of rat Neuregulin-2 (Accession O35569). Extracellular, N-terminus.
Accession (Uniprot) Number O35569
Gene ID 432361
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    1,2. Anti-NRG2 (extracellular) Antibody (#ANR-114), (1:200).
    3,4. Anti-NRG2 (extracellular) Antibody, preincubated with NRG2 (extracellular) Blocking Peptide (#BLP-NR114).
Immunohistochemistry
  • Expression of Neuregulin-2 in mouse hippocampus and cerebellum
    Expression of Neuregulin-2 in mouse hippocampus and cerebellum
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-NRG2 (extracellular) Antibody (#ANR-114), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. NRG2 staining in CA1 hippocampal region (green) is detected in the pyramidal layer (P, arrows). B. Staining in the cerebellum (green) is observed in Purkinje cells (arrows). Mol= Molecular layer; GL= Granular layer. Cell nuclei are stained with DAPI (blue).
References
  1. Avramopoulos, D. (2018) Am. J. Med. Genet. B Neuropsychiatr. Genet. 177, 257.
  2. Centa, A. et al. (2018) Mol. Oncol. 12, 1061.
  3. Yan, L. et al. (2018) Mol. Psychiatry 23, 1233.
Scientific background

Neuregulins (NRGs) are a multi member protein family involved in cell to cell communication. This family includes four human neuregulin paralogs NRG1-NRG4 which produce multiple isoforms through alternative splicing.

NRGs are involved in several physiological processes including heart and peripheral nervous system development. They act in part by binding to the extracellular region of ErbB/HER transmembrane tyrosine kinases, leading to receptor dimerization and ultimately to the activation of a complex network of downstream signaling events1,2.

NRG2 transcript levels markedly increase following birth, suggesting that NRG2 has important functions in the postnatal and adult brain. Several studies have also shown that NRG2 modulates glutamatergic transmission in GABAergic neurons and selectively affects GluN2B-containing NMDA receptors2,3.

The region containing the NRG2 locus is associated with schizophrenia and other psychiatric disorders and genetically linked to ADHD in genome-wide scans2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-NRG2 (extracellular) Antibody (#ANR-114) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Neuregulin-2 from rat, mouse, and human samples.

For research purposes only, not for human use
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