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Anti-Orai2 Antibody

TMEM142B

Cat #: ACC-061
Alternative Name TMEM142B
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CPEPGHKGMDYRDWVRR, corresponding to amino acid residues 16-32 of mouse Orai2 (Accession Q8BH10). Intracellular, N-terminus.
Accession (Uniprot) Number Q8BH10
Gene ID 269717
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human, rat - identical.
RRID AB_2040046.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of human HL-60 promyelocytic leukemia (lanes 1 and 3) and human Jurkat acute T cell leukemia (lanes 2 and 4) cell lysates:
    Western blot analysis of human HL-60 promyelocytic leukemia (lanes 1 and 3) and human Jurkat acute T cell leukemia (lanes 2 and 4) cell lysates:
    1,2. Anti-Orai2 Antibody (#ACC-061), (1:200).
    3,4. Anti-Orai2 Antibody, preincubated with Orai2 Blocking Peptide (#BLP-CC061).
Immunohistochemistry
  • Expression of Orai2 in rat lung
    Expression of Orai2 in rat lung
    Immunohistochemical staining of paraffin-embedded rat lung sections using Anti-Orai2 Antibody (#ACC-061), (1:100). Strong and specific staining is evident in bronchiolar epithelium (blue) and alveoli walls (red). Hematoxilin is used as the counterstain.
References
  1. Eisner, D.A. et al. (2005) Exp. Physiol. 90, 3.
  2. Chakrabarti, R. and Chakrabarti, R. (2006) J. Cell. Biochem. 99, 1503.
  3. Feske, S. et al. (2006) Nature 441, 179.
  4. Pickett, J. (2006) Nature Reviews Mol. Cell Biol. 7, 794.
  5. DeHaven, W.I. et al. (2007) J. Biol. Chem. 282, 17548.
  6. Mercer, J.C. et al. (2006) J. Biol. Chem. 281, 24979.
  7. Gwack, Y. et al. (2007) J. Biol. Chem. 282, 16232.
Scientific background

Cytosolic Ca2+ has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, and cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx, either by the calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores, has become of great interest.

Recently, several key players of the store-operated complex have been identified: the Orai family consisting of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2.3 Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum Ca2+ sensor.3,4 Orai1, Orai2, and Orai3 are all capable of forming store-operated channels.5

Co-expresssion of Orai2 and STIM1 was shown to produce currents that appear similar but smaller than those produced by co-expression of Orai1 and STIM1.6

Orai2 transcripts are predominantly expressed in kidney, lung, and spleen, and it appears that Orai2 is the only Orai isoform with multiple transcripts.7

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title:

Anti-Orai2 Antibody
Expression of Orai2 in human kidney.Immunohistochemical staining of human kidney sections using Anti-Orai2 Antibody (#ACC-061). In paraffin-embedded kidney sections, Orai2 is localized to kidney tubules, with stronger staining in the proximal tubules (upper left panel). Antibody preabsorbed with the blocking peptide (supplied with the antibody) was used as control to show the specificity of the antibody (lower left panel). In frozen kidney sections Orai2 staining is observed in renal tubules (upper right panel). Smooth muscle actin staining is shown in red (lower right panel).Adapted from Zeng, B. et al. (2017) Nat. Commun. 8, 1920. with permission of SPRINGER NATURE.

Last update: 06/09/2021

Anti-Orai2 Antibody (#ACC-061) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Orai2 from human, mouse, and rat samples.

For research purposes only, not for human use

Applications

Citations

Citations
Western blot citations
  1. HeLa cell lysate (1:200).
    Bittremieux, M. et al. (2017) Cell Calcium 62, 60.
  2. Mouse PA smooth muscle lysate (1:500).
    Fernandez, R.A. et al. (2015) Am. J. Physiol. 308, C581.
Immunohistochemistry citations
  1. Mouse brain sections (1:500).
    Kikuta, S. et al. (2019) Front. Cell. Neurosci. 13, 547.
  2. Human kidney sections.
    Zeng, B. et al. (2017) Nat. Commun. 8, 1920.
Immunofluorescence citations
  1. Mouse brain sections (1:500).
    Kikuta, S. et al. (2019) Front. Cell. Neurosci. 13, 547.
More product citations
  1. Mitchell, C.M. et al. (2012) J. Neurochem. 122, 1155.

Specifications

Scientific Background

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