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Anti-P2X3 Receptor (extracellular) Antibody

P2RX3, P2X purinoceptor 3

Cat #: APR-026
Alternative Name P2RX3, P2X purinoceptor 3
  • KO Validated
    • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
    Immunogen
    • Peptide (C)KGFGRYANRVMDVSD, corresponding to amino acid residues 65-79 of rat P2X3 receptor (Accession P49654). Extracellular.
    Accession (Uniprot) Number P49654
    Gene ID 81739
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – identical; human - 13/15 amino acid residues identical.
    RRID AB_2341052.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ifc, ih, lci, wb
    May also work in: ip*
    Western blot
    • Western blot analysis of rat DRG:
      Western blot analysis of rat DRG:
      1. Anti-P2X3 Receptor (extracellular) Antibody (#APR-026), (1:200).
      2. Anti-P2X3 Receptor (extracellular) Antibody, preincubated with P2X3 Receptor (extracellular) Blocking Peptide (#BLP-PR026).
    Immunohistochemistry
    • Expression of P2RX3 in rat DRG
      Expression of P2RX3 in rat DRG
      Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-P2X3 Receptor (extracellular) Antibody (#APR-026), followed by anti-rabbit-AlexaFluor-594 secondary antibody. P2RX3 staining (red) appears in neuronal cell bodies. DAPI was used as the counter stain (blue). A. X20 magnification. B. X40 magnification.
    Indirect flow cytometry
    • Cell surface detection of P2X3 Receptor by indirect flow cytometry in live intact mouse P815 mastocytoma cells:
      Cell surface detection of P2X3 Receptor by indirect flow cytometry in live intact mouse P815 mastocytoma cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells +  Anti-P2X3 Receptor (extracellular) Antibody (#APR-026), (2.5μg) + goat-anti-rabbit-FITC.
    Live cell imaging / Immunocytochemistry
    • Expression of P2RX3 in rat PC12 cells
      Expression of P2RX3 in rat PC12 cells
      Cell surface detection of P2RX3 in intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining with Anti-P2X3 Receptor (extracellular) Antibody (#APR-026), (1:50, red) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. B. DAPI was used as the counter stain (blue). C. Merge images of A and B.
    References
    1. Florenzano, F. et al. (2002) Neuroscience 115, 425.
    2. Ashcroft, F.M. et al. (1999) Ion Channels and Disease Ed.1.
    3. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107.
    4. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
    5. Kage, K. et al. (2002) Exp. Brain Res. 147, 511.
    6. Prasad, M. et al. (2001) J. Physiol. 573, 667.
    7. Aoki, Y. et al. (2003) Brain Res. 989, 214.
    8. Burnstock, G. (2000) Br. J. Anaesth. 84, 476.
    Scientific background

    The P2X3 receptor belongs to the ligand-gated ion channel P2X receptor family, that consists of seven receptor subtypes named P2X1-P2X7 and is activated by extracellular ATP.1,2,3

    All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.4-5 The different P2X receptors show distinct expression patterns. P2X1-6 has been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system. The P2X3 receptor is highly expressed on nociceptive sensory neurons in dorsal root ganglia (DRG) as a homomer or as a heteromer (P2X3/P2X2). ATP released from damaged cells activates the P2X3 receptor to initiate nociceptive signals.6,7 Involvement of ATP in the mechanism of chronic pain has been also suggested.7,8 P2X3 receptor is now becoming a possible target for the development of pain therapeutics.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Lyophilized Powder
    Last update: 02/03/2021

    Anti-P2X3 Receptor (extracellular) Antibody (#APR-026) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and live cell imaging applications. It has been designed to recognize P2X3 receptor from rat, mouse, and human samples.

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    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    KO validation citations
    1. Western blot and immunohistochemistry of rat stellate ganglia. Tested in P2X3 shRNA knockdown rats. 
      Sheng, X.       et al. (2018) Neurochem. Int. 120, 206.
    Western blot citations
    1. Rat DRG lysate (1:2000).
      Gu, Y. et al. (2016) Mol. Pain 12, 1.
    Immunocytochemistry citations
    1. Rat DRGs (1:100).
      Gu, Y. et al. (2016) Mol. Pain 12, 1.

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