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Anti-P2X5 Receptor (extracellular) Antibody

P2RX5, P2X purinoceptor 5
Cat #: APR-027
Alternative Name P2RX5, P2X purinoceptor 5
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)HRSHTRLAEMKYE, corresponding to amino acid residues 299-311 of rat P2X5 receptor (Accession Q6P719).
Accession (Uniprot) Number Q6P719
Gene ID 303211
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse –12/13 amino acid residues; human – 10/13 amino acid residues.
RRID AB_2756757.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat heart (lanes 1 and 3) and mouse heart (lanes 2 and 4):
    Western blot analysis of rat heart (lanes 1 and 3) and mouse heart (lanes 2 and 4):
    1,2. Anti-P2X5 Receptor (extracellular) Antibody (#APR-027), (1:400).
    3,4. Anti-P2X5 Receptor (extracellular) Antibody, preincubated with P2X5 Receptor (extracellular) Blocking Peptide (#BLP-PR027).
References
  1. Prasad, M. et al. (2001) J. Physiol. 537, 667.
  2. Florenzano, F. et al. (2002) Neuroscience 115, 425.
  3. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107.
  4. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289.
  5. Torres, G.E. et al. (1998) Mol. Pharmacol. 54, 989.
  6. Greig, A.V. et al. (2003) J. Invest. Dermatol. 121, 1145.
  7. Greig, A.V. et al. (2003) J. Invest. Dermatol. 120, 1007.
  8. Slater, M. et al. (2000) Histochem. J. 32, 571.
  9. Afework, M. and Burnstock, G. (2000) Int. J. Dev. Neurosci. 18, 515.
  10. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed.1.
Scientific background

The P2X5 receptor (P2X5R) belongs to the ligand-gated ion channel P2X family, which is activated by extracellular ATP. To date, seven receptor subtypes named P2X1-P2X7 have been described.1,2,3  

All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.4-5 The P2X5R forms a heteromeric receptor with P2X1 receptor. The heteromeric receptor has distinct properties from the P2X1 or P2X5 homomeric receptors.4,6 The different P2X receptors show distinct expression patterns.  

P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and in microglia.  

The P2X5 subunit was first cloned from rat heart, and was reported to be expressed in heart, brain, spinal cord and adrenal gland. 

Recent papers suggested possible roles for P2X5 receptor in differentiation and apoptosis in keratinocytes and ageing rat prostate, respectively.7-10

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat P2X5 receptor. Anti-P2X5 Receptor (extracellular) Antibody (#APR-027) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal to detect the receptor in living cells. It has been designed to recognize P2RX5 from mouse, rat and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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