Anti-P2X5 Receptor Antibody

P2RX5, P2X purinoceptor 5
Cat #: APR-005
Alternative Name P2RX5, P2X purinoceptor 5
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, r
    May also work in: m
      • Peptide (C)RENAIVNVKQSQILHPVKT, corresponding to amino acid residues 437-455 of rat P2X5 receptor (Accession P51578). Intracellular, C-terminus.
    Accession (Uniprot) Number P51578
    Gene ID 113995
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 13/18 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, ih, wb
    May also work in: ic, ip
      • Western blot analysis of rat brain membranes:
        1. Anti-P2X5 Receptor Antibody (#APR-005), (1:200). 
        2. Anti-P2X5 Receptor Antibody, preincubated with the control peptide antigen.
      • Expression of P2RX5 in rat spinal cord
        Immunohistochemical staining of rat spinal cord frozen section using Anti-P2X5 Receptor Antibody (#APR-005), (1:100). P2RX5 staining (red) is specific for neurons in the anterior horn of the spinal cord. Hoechst 33342 is used as the counterstain.
      • Human CD4+ and ckit+ cells (Kazakova, R.R. et al. (2011) Bull. Exp. Biol. Med. 151, 33.).
      • The P2X5 receptor (P2RX5) belongs to the ligand-gated ion channel P2X family, which is activated by extracellular ATP. To date, seven receptor subtypes named P2X1-P2X7 have been described.1,2,3  

        All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.4-5 The P2RX5 forms a heteromeric receptor with P2X1 receptor. The heteromeric receptor has distinct properties from the P2X1 or P2X5 homomeric receptors.4,6 The different P2X receptors show distinct expression patterns.  

        P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and in microglia.  

        The P2X5 subunit was first cloned from rat heart, and was reported to be expressed in heart, brain, spinal cord and adrenal gland. 

        Recent papers suggested possible roles for P2X5 receptor in differentiation and apoptosis in keratinocytes and ageing rat prostate, respectively.7-10

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 11/06/2019

    Alomone Labs offers a highly specific antibody directed against an intracellular epitope at the C-terminus of the rat P2X5 receptor. Anti-P2X5 Receptor Antibody (#APR-005) can be used in western blot, immunocytochemistry, immunohistochemistry, immunoprecipitation and indirect flow cytometry applications. It has been designed to recognize P2RX5 from rat, mouse and human samples.

    For research purposes only, not for human use