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Anti-P2Y1 Receptor (extracellular) Antibody

P2Y purinoceptor 1, P2RY1

Cat #: APR-021
Alternative Name P2Y purinoceptor 1, P2RY1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide SDEYLRSYFIYSMC, corresponding to amino acid residues 207-220 of human P2RY1 (Accession P47900). 2nd extracellular loop.
Accession (Uniprot) Number P47900
Gene ID 5028
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - identical; mouse - 12/14 amino acid residues identical.
RRID AB_10919250.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ih, lci, wb
May also work in: ip*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 4), Jurkat (lanes 2 and 5) and K-562 (lanes 3 and 6) lysates:
    Western blot analysis of rat brain (lanes 1 and 4), Jurkat (lanes 2 and 5) and K-562 (lanes 3 and 6) lysates:
    1-3. Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021), (1:200).
    4-6. Anti-P2Y1 Receptor (extracellular) Antibody, preincubated with P2Y1 Receptor (extracellular) Blocking Peptide (#BLP-PR021).
Immunohistochemistry
  • Expression of P2RY1 in mouse hippocampus
    Expression of P2RY1 in mouse hippocampus
    Immunohistochemical staining of mouse hippocampal CA3 region using Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021). A. P2RY1 staining (red) appears in the pyramidal layer (arrows). B. Nuclei staining using DAPI as the counterstain (blue). C. Merged images of panels A and B.
Indirect flow cytometry
  • Cell surface detection of P2RY1 in Jurkat (human acute T cell leukemia) living cells:
    Cell surface detection of P2RY1 in Jurkat (human acute T cell leukemia) living cells:
    ___ Unstained cells.
    ___ Cells stained + Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021), (5 μg/1x106 cells).
  • The control antigen is not suitable for this application.
Live cell imaging / Immunocytochemistry
  • Expression of P2RY1 in rat PC12 cells
    Expression of P2RY1 in rat PC12 cells
    Cell surface detection of P2RY1 in intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Merge of extracellular staining with live view of the cells.
References
  1. Wang, L. et al. (2002) J. Cardiovas. Pharmacol. 40, 841.
  2. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
  3. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
  4. Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
  5. Jin, J. et al. (1998) J. Biol. Chem. 273, 2030.
Scientific background

The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiologic responses, among them, cardiac function, platelet aggregation and SMC proliferation.1

The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4

P2Y1 receptor is abundantly expressed in a number of tissues. In platelets, P2Y1 receptor is co-expressed with P2Y12 receptor, responsible for the initial shape change, aggregation, and rise in intracellular Ca2+ upon activation in response to ADP.5

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Last update: 21/12/2020

Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021) is a highly specific antibody directed against an extracellular epitope of the human P2RY1. The antibody can be used in western blot, immunohistochemistry, indirect flow cytometry and live cell imaging applications. It has been designed to recognize P2RY1 from mouse, rat and human samples.

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For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Immunohistochemistry citations
  1. Mouse DRG Sections (1:650).
    Mitchell, R. et al. (2019) Mol. Neurobiol. 56, 5917.
Immunofluorescence citations
  1. Mouse DRG Sections (1:650).
    Mitchell, R. et al. (2019) Mol. Neurobiol. 56, 5917.
Indirect flow cytometry citations
  1. Mouse primary microglia culture.
    Fukumoto, Y. et al. (2018) J. Cereb. Blood Flow Metab. 39, 2144.

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