Anti-P2Y1 Receptor (extracellular) Antibody

P2Y purinoceptor 1, P2RY1
    Cat #: APR-021
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide SDEYLRSYFIYSMC, corresponding to amino acid residues 207-220 of human P2RY1 (Accession P47900). 2nd extracellular loop.
    Accession (Uniprot) Number 5028
    Gene ID P47900
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - identical; mouse - 12/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ifc, ih, lci, wb
    May also work in: ip
    Western blot
    Western blot analysis of rat brain (lanes 1 and 4), Jurkat (lanes 2 and 5) and K-562 (lanes 3 and 6) lysates:
    1-3. Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021), (1:200).
    4-6. Anti-P2Y1 Receptor (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of P2RY1 in mouse hippocampus
    Immunohistochemical staining of mouse hippocampal CA3 region using Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021). A. P2RY1 staining (red) appears in the pyramidal layer (arrows). B. Nuclei staining using DAPI as the counterstain (blue). C. Merged images of panels A and B.
    Indirect flow cytometry
    Indirect flow cytometry analysis of Jurkat (human acute T cell leukemia) living cells:
    ___ Unstained cells.
    ___ Cells stained + Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021), (5 μg/1x106 cells).
    The control antigen is not suitable for this application.
    Live cell imaging / Immunocytochemistry
    Expression of P2RY1 in rat PC12 cells
    Immunocytochemical staining of intact living rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Merge of extracellular staining with live view of the cells.
    References
    1. Wang, L. et al. (2002) J. Cardiovas. Pharmacol40, 841.
    2. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther307, 809.
    3. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev50, 413.
    4. Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci24, 52.
    5. Jin, J. et al. (1998) J. Biol. Chem273, 2030.
    Scientific background

    The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiologic responses, among them, cardiac function, platelet aggregation and SMC proliferation.1

    The mammalian P2Y family currently includes eight functional receptors; P2Y1P2Y2P2Y4P2Y6P2Y11P2Y12P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4

    P2Y1 receptor is abundantly expressed in a number of tissues. In platelets, P2Y1 receptor is co-expressed with P2Y12 receptor, responsible for the initial shape change, aggregation, and rise in intracellular Ca2+ upon activation in response to ADP.5

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 04/12/2018

    Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021) is a highly specific antibody directed against an extracellular epitope of the human P2RY1. The antibody can be used in western blot, immunohistochemistry, indirect flow cytometry and live cell imaging applications. It has been designed to recognize P2RY1 from mouse, rat and human samples.

    For research purposes only, not for human use
    Citations
    Related Products
      • Products for control experiments
        1. Anti-P2Y1 Receptor Antibody (#APR-009).
          This antibody recognizes a different epitope and thus can also be used as a control for detecting P2Y1.