- Western blot analysis of rat brain membranes (lanes 1 and 3) and human platelets lysate (lanes 2 and 4):1,2. Anti-P2Y1 Receptor Antibody (#APR-009), (1:200).
3,4. Anti-P2Y1 Receptor Antibody, preincubated with P2Y1 Receptor Blocking Peptide (#BLP-PR009). - Mouse spinal cord astrocyte lysate (1:200) (Suadicani, S.O. et al. (2003) Glia 42, 160.).
- Rat cortical neuron lysate (4 μg) (Siow, N.L. et al. (2010) Mol. Pharmacol. 78, 1059.).
- Expression of P2RY1 in rat thalamusImmunohistochemical staining of fasciculi in rat thalamus using Anti-P2Y1 Receptor Antibody (#APR-009). Specific staining is black. Cresyl violet is used as the counterstain.
- Human eye tissue (Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.).
- Rat primary hippocampal neurons (1:100) (Filippov, A.K. et al. (2006) J. Neurosci. 26, 9340.).
- Rat osteoclasts (6 μg/ml) (Alvarenga, E.C. et al. (2010) Bone 46, 355.).
- The blocking peptide is not suitable for this application.
- Wang, L. et al. (2002) J. Cardiovas. Pharmacol. 40, 841.
- Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
- Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
- Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
- Jin, J. et al. (1998) J. Biol. Chem. 273, 2030.
The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiologic responses, among them, cardiac function, platelet aggregation and SMC proliferation.1
The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4
P2Y1 receptor is abundantly expressed in a number of tissues. In platelets, P2Y1 receptor is co-expressed with P2Y12 receptor, responsible for the initial shape change, aggregation, and rise in intracellular Ca2+ upon activation in response to ADP.5
Application key:
Species reactivity key:
Expression of P2Y1 receptor in mouse longitudinal muscle-myenteric plexus sections.Immunohistochemical staining of mouse longitudinal muscle-myenteric plexus sections using Anti-P2Y1 Receptor Antibody (#APR-009). P2Y1 receptor staining (yellow) is detected in myenteric neurons.
Adapted from Caputi, V. et al. (2017) Front. Pharmacol. 8, 350. with permission of Frontiers.
Anti-P2Y1 Receptor Antibody (#APR-009) is a highly specific antibody directed against an epitope of the human P2RY1. The antibody can be used in western blot, indirect flow cytometry, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize P2RY1 from human, rat, and mouse samples.
Applications
Citations
- Western blot analysis of mouse muscle tissue lysate. Tested in P2Y1-/- mice.
Xu, M.L. et al. (2015) J. Mol. Neurosci. 57, 446.
- Mouse brain lysate.
Cui, J.D. et al. (2016) Chem. Biol. Interact. 259, 291. - Mouse bone marrow lysate.
Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29. - Mouse bone lysate and osteoblast MOB-C cell lysate.
Seref-Ferlengez, Z. et al. (2016) PLoS ONE 11, e0155107. - Mouse muscle tissue lysate. Also tested in P2Y1-/- mice.
Xu, M.L. et al. (2015) J. Mol. Neurosci. 57, 446. - Rat heart lysate (1:200).
Giachini, F.R. et al. (2014) PLoS ONE 9, e91890. - Mouse spinal cord astrocyte lysate (1:200).
Suadicani, S.O. et al. (2003) Glia 42, 160.
- Mouse bone marrow lysate.
Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29. - Rat cortical neuron lysate (4 μg).
Siow, N.L. et al. (2010) Mol. Pharmacol. 78, 1059.
- Mouse muscle-myenteric plexus sections.
Caputi, V. et al. (2017) Front. Pharmacol. 8, 350. - Mouse brain sections (1:100).
Ribeiro Xavier, A.L. et al. (2015) J. Neurosci. 35, 11848. - Human eye tissue.
Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.
- Human fibroblasts (1:50).
Pinheiro, A.R. et al. (2013) J. Biol. Chem. 288, 27571. - Rat primary hippocampal neurons (1:100).
Filippov, A.K. et al. (2006) J. Neurosci. 26, 9340.
- Human blood samples.
Ngo, A.T.P. et al. (2020) Platelets 131, 68. - Mouse bone marrow cells.
Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29. - Rat osteoclasts (6 μg/ml).
Alvarenga, E.C. et al. (2010) Bone 46, 355.
- Frobel, J. et al. (2013) Mol. Cell. Proteomics 12, 1272.
- Zerpa, H. et al. (2013) Eur. J. Pharmacol. 702, 242.
Specifications
Scientific Background
Related Products
- Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021).
This antibody recognizes a different epitope and thus can also be used as a control for detecting P2Y1.
- Anti-P2Y1 Receptor (extracellular)-ATTO Fluor-488 Antibody (#APR-021-AG).
A fluorescent labeled primary antibody. It can be used in multiplex staining studies in conjunction with any of our antibodies raised in rabbit.
- Anti-P2Y1 Receptor (extracellular) Antibody (#APR-021). This antibody recognizes an extracellular epitope and can be used to detect P2Y1 in live cells.
Antibodies
- Anti-P2Y1 Receptor (extracellular)-FITC Antibody (#APR-021-F)
- Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody (#APR-012-AR)
- Anti-P2Y13 Receptor Antibody (#APR-017)
- Anti-P2Y14 Receptor (extracellular) Antibody (#APR-018)
- Anti-P2Y2 Receptor Antibody (#APR-010)
- Anti-P2Y4 Receptor Antibody (#APR-006)
- Anti-P2Y6 Receptor Antibody (#APR-011)
Explorer kits & Research packs
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