Anti-P2Y1 Receptor Antibody

P2Y purinoceptor 1, P2RY1
    Cat #: APR-009
    Alternative Name P2Y purinoceptor 1, P2RY1
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)RALI YKDLD NSPLR RKS, corresponding to residues 242-258 of rat or human P2RY1. (Accession P49651P47900). 3rd intracellular loop (i3) between TM5 and TM6 domains.
    • Anti-P2Y1 Receptor Antibody
    Accession (Uniprot) Number P49651
    Gene ID 25265
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, human, bovine - identical; turkey, chicken - 16/17 amino acid residues identical; mouse - 15/17 amino acid residues identical.
    RRID AB_2040070.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ifc, ih, ip, wb
    Western blot
    • Anti-P2Y1 Receptor Antibody
      Western blot analysis of rat brain membranes (lanes 1, 3) and human platelets lysate (lanes 2, 4):
      1,2. Anti-P2Y1 Receptor Antibody (#APR-009), (1:200).
      3,4. Anti-P2Y1 Receptor Antibody, preincubated with the negative control antigen.
    • Mouse spinal cord astrocyte lysate (1:200) (Suadicani, S.O. et al. (2003) Glia 42, 160.).
    Immunoprecipitation
    • Rat cortical neuron lysate (4 μg) (Siow, N.L. et al. (2010) Mol. Pharmacol. 78, 1059.).
    Immunohistochemistry
    • Anti-P2Y1 Receptor Antibody
      Expression of P2RY1 in rat thalamus
      Immunohistochemical staining of fasciculi in rat thalamus using Anti-P2Y1 Receptor Antibody (#APR-009). Specific staining is black. Cresyl violet is used as the counterstain.
    • Human eye tissue (Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.).
    Immunocytochemistry
    • Rat primary hippocampal neurons (1:100) (Filippov, A.K. et al. (2006) J. Neurosci. 26, 9340.).
    Indirect flow cytometry
    • Rat osteoclasts (6 μg/ml) (Alvarenga, E.C. et al. (2010) Bone 46, 355.).
    • The negative control antigen is not suitable for this application.
    References
    1. Wang, L. et al. (2002) J. Cardiovas. Pharmacol. 40, 841.
    2. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
    3. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
    4. Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
    5. Jin, J. et al. (1998) J. Biol. Chem. 273, 2030.
    Scientific background

    The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiologic responses, among them, cardiac function, platelet aggregation and SMC proliferation.1

    The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2P2Y4P2Y6P2Y11P2Y12P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4

    P2Y1 receptor is abundantly expressed in a number of tissues. In platelets, P2Y1 receptor  is co-expressed with P2Y12 receptor,  responsible for the initial shape change, aggregation, and rise in intracellular Ca2+ upon activation in response to ADP.5

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-P2Y1 Receptor Antibody
    Expression of P2Y1 receptor in mouse longitudinal muscle-myenteric plexus sections.Immunohistochemical staining of mouse longitudinal muscle-myenteric plexus sections using Anti-P2Y1 Receptor Antibody (#APR-009). P2Y1 receptor staining (yellow) is detected in myenteric neurons.
    Adapted from Caputi, V. et al. (2017) Front. Pharmacol. 8, 350. with permission of Frontiers.
    Last update: 24/01/2020

    Anti-P2Y1 Receptor Antibody (#APR-009) is a highly specific antibody directed against an epitope of the human P2RY1. The antibody can be used in western blot, indirect flow cytometry, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize P2RY1 from human, rat, and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    KO validation citations
    1. Western blot analysis of mouse muscle tissue lysate. Tested in P2Y1-/- mice.
      Xu, M.L. et al. (2015) J. Mol. Neurosci. 57, 446.
    Western blot citations
    1. Mouse brain lysate.
      Cui, J.D. et al. (2016) Chem. Biol. Interact. 259, 291.
    2. Mouse bone marrow lysate.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    3. Mouse bone lysate and osteoblast MOB-C cell lysate.
      Seref-Ferlengez, Z. et al. (2016) PLoS ONE 11, e0155107.
    4. Mouse muscle tissue lysate. Also tested in P2Y1-/- mice.
      Xu, M.L. et al. (2015) J. Mol. Neurosci. 57, 446.
    5. Rat heart lysate (1:200).
      Giachini, F.R. et al. (2014) PLoS ONE 9, e91890.
    6. Mouse spinal cord astrocyte lysate (1:200).
      Suadicani, S.O. et al. (2003) Glia 42, 160.
    Immunoprecipitation citations
    1. Mouse bone marrow lysate.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    2. Rat cortical neuron lysate (4 μg).
      Siow, N.L. et al. (2010) Mol. Pharmacol. 78, 1059.
    Immunohistochemistry citations
    1. Mouse muscle-myenteric plexus sections.
      Caputi, V. et al. (2017) Front. Pharmacol. 8, 350.
    2. Mouse brain sections (1:100).
      Ribeiro Xavier, A.L. et al. (2015) J. Neurosci. 35, 11848.
    3. Human eye tissue.
      Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.
    Immunocytochemistry citations
    1. Human fibroblasts (1:50).
      Pinheiro, A.R. et al. (2013) J. Biol. Chem. 288, 27571.
    2. Rat primary hippocampal neurons (1:100).
      Filippov, A.K. et al. (2006) J. Neurosci. 26, 9340.
    Indirect flow cytometry citations
    1. Mouse bone marrow cells.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    2. Rat osteoclasts (6 μg/ml).
      Alvarenga, E.C. et al. (2010) Bone 46, 355.
    More product citations
    1. Frobel, J. et al. (2013) Mol. Cell. Proteomics 12, 1272.
    2. Zerpa, H. et al. (2013) Eur. J. Pharmacol. 702, 242.
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