Overview
Cat #:
APR-009
Alternative Name P2Y purinoceptor 1, P2RY1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
Accession (Uniprot) Number P49651
Gene ID 25265
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human, bovine - identical; turkey, chicken - 16/17 amino acid residues identical; mouse - 15/17 amino acid residues identical.
RRID AB_2040070.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ih, ip, wb
Western blot
Western blot analysis of rat brain membranes (lanes 1 and 3) and human platelets lysate (lanes 2 and 4):1,2. Anti-P2Y1 Receptor Antibody (#APR-009), (1:200).
3,4. Anti-P2Y1 Receptor Antibody, preincubated with P2Y1 Receptor Blocking Peptide (#BLP-PR009).- Mouse spinal cord astrocyte lysate (1:200) (Suadicani, S.O. et al. (2003) Glia 42, 160.).
Immunoprecipitation
- Rat cortical neuron lysate (4 μg) (Siow, N.L. et al. (2010) Mol. Pharmacol. 78, 1059.).
Immunohistochemistry
Expression of P2RY1 in rat thalamusImmunohistochemical staining of fasciculi in rat thalamus using Anti-P2Y1 Receptor Antibody (#APR-009). Specific staining is black. Cresyl violet is used as the counterstain.- Human eye tissue (Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.).
Expression of P2RY1 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-P2Y1 Receptor Antibody (#APR-009), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the hippocampal dentate gyrus region, showed immunoreactivity (green) in in the sub-granular layer (arrows) and diffusely in the outer molecular layer (OML). B. Pre-incubation of the antibody with P2Y1 Receptor Blocking Peptide (#BLP-PR009), suppressed staining. Cell nuclei are stained with DAPI (blue). G = granule layer, OML = outer molecular layer.- Expression of P2RY1 in rat spinal cord.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-P2Y1 Receptor Antibody (#APR-009), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. P2Y1 immunoreactivity (green) appears in layers 1-2 of the dorsal horn (arrows). B. Pre-incubation of the antibody with P2Y1 Receptor Blocking Peptide (#BLP-PR009), suppressed staining. Cell nuclei are stained with DAPI (blue).
Immunocytochemistry
- Rat primary hippocampal neurons (1:100) (Filippov, A.K. et al. (2006) J. Neurosci. 26, 9340.).
Indirect flow cytometry
- Rat osteoclasts (6 μg/ml) (Alvarenga, E.C. et al. (2010) Bone 46, 355.).
- The blocking peptide is not suitable for this application.
References
- Wang, L. et al. (2002) J. Cardiovas. Pharmacol. 40, 841.
- Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
- Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
- Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
- Jin, J. et al. (1998) J. Biol. Chem. 273, 2030.
Scientific background
The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiologic responses, among them, cardiac function, platelet aggregation and SMC proliferation.1
The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4
P2Y1 receptor is abundantly expressed in a number of tissues. In platelets, P2Y1 receptor is co-expressed with P2Y12 receptor, responsible for the initial shape change, aggregation, and rise in intracellular Ca2+ upon activation in response to ADP.5
Lyophilized Powder
Anti-P2Y1 Receptor Antibody (#APR-009) is a highly specific antibody directed against an epitope of the human P2RY1. The antibody can be used in western blot, indirect flow cytometry, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize P2RY1 from human, rat, and mouse samples.
Image & Title: 
Expression of P2Y1 receptor in mouse longitudinal muscle-myenteric plexus sections.Immunohistochemical staining of mouse longitudinal muscle-myenteric plexus sections using Anti-P2Y1 Receptor Antibody (#APR-009). P2Y1 receptor staining (yellow) is detected in myenteric neurons.
Adapted from Caputi, V. et al. (2017) Front. Pharmacol. 8, 350. with permission of Frontiers.
Expression of P2Y1 receptor in mouse longitudinal muscle-myenteric plexus sections.Immunohistochemical staining of mouse longitudinal muscle-myenteric plexus sections using Anti-P2Y1 Receptor Antibody (#APR-009). P2Y1 receptor staining (yellow) is detected in myenteric neurons.
Adapted from Caputi, V. et al. (2017) Front. Pharmacol. 8, 350. with permission of Frontiers.
For research purposes only, not for human use
Last Update: 13/04/2023
Applications
Citations
Citations
Powered by BiozKO validation citations
- Western blot analysis of mouse muscle tissue lysate. Tested in P2Y1-/- mice.
Xu, M.L. et al. (2015) J. Mol. Neurosci. 57, 446.
Western blot citations
- Mouse brain lysate.
Cui, J.D. et al. (2016) Chem. Biol. Interact. 259, 291. - Mouse bone marrow lysate.
Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29. - Mouse bone lysate and osteoblast MOB-C cell lysate.
Seref-Ferlengez, Z. et al. (2016) PLoS ONE 11, e0155107. - Mouse muscle tissue lysate. Also tested in P2Y1-/- mice.
Xu, M.L. et al. (2015) J. Mol. Neurosci. 57, 446. - Rat heart lysate (1:200).
Giachini, F.R. et al. (2014) PLoS ONE 9, e91890. - Mouse spinal cord astrocyte lysate (1:200).
Suadicani, S.O. et al. (2003) Glia 42, 160.
Immunoprecipitation citations
- Mouse bone marrow lysate.
Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29. - Rat cortical neuron lysate (4 μg).
Siow, N.L. et al. (2010) Mol. Pharmacol. 78, 1059.
Immunohistochemistry citations
- Mouse muscle-myenteric plexus sections.
Caputi, V. et al. (2017) Front. Pharmacol. 8, 350. - Mouse brain sections (1:100).
Ribeiro Xavier, A.L. et al. (2015) J. Neurosci. 35, 11848. - Human eye tissue.
Fries, J.E. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 3000.
Immunocytochemistry citations
- Human fibroblasts (1:50).
Pinheiro, A.R. et al. (2013) J. Biol. Chem. 288, 27571. - Rat primary hippocampal neurons (1:100).
Filippov, A.K. et al. (2006) J. Neurosci. 26, 9340.
Indirect flow cytometry citations
- Human blood samples.
Ngo, A.T.P. et al. (2020) Platelets 131, 68. - Mouse bone marrow cells.
Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29. - Rat osteoclasts (6 μg/ml).
Alvarenga, E.C. et al. (2010) Bone 46, 355.
More product citations
- Frobel, J. et al. (2013) Mol. Cell. Proteomics 12, 1272.
- Zerpa, H. et al. (2013) Eur. J. Pharmacol. 702, 242.
