Anti-P2Y12 Receptor Antibody

P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3
    Cat #: APR-012
    Alternative Name P2Y purinoceptor 12, P2RY12, ADP-glucose receptor, Platelet ADP receptor, HORK3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)KTTRPFKTSNPKNLLGAK,  corresponding to amino acid residues 125-142 of human P2RY12 (Accession Q9H244). Intracellular, 2nd cytoplasmic loop.
    • Anti-P2Y12 Receptor Antibody
    Accession (Uniprot) Number Q9H244
    Gene ID 64805
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - 16/18 amino acid residues identical.
    RRID AB_2040074.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ifc, ih, ip, wb
    Western blot
    • Anti-P2Y12 Receptor Antibody
      Western blot analysis of rat brain membranes (1, 2) and human platelets (3, 4): 
      1,3. Anti-P2Y12 Receptor Antibody (#APR-012), (1:200).   
      2,4. Anti-P2Y12 Receptor Antibody, preincubated with the negative control antigen.
    • Anti-P2Y12 Receptor Antibody
      Western blot analysis of human platelets:
      1. Anti-P2Y12 Receptor Antibody (#APR-012), (1:200)
      2. Anti-P2Y12 Receptor Antibody, preincubated with the negative control antigen.
    Immunoprecipitation
    • Mouse bone marrow lysate (Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.).
    Immunohistochemistry
    • Anti-P2Y12 Receptor Antibody
      Expression of P2RY12 in mouse brain
      Immunohistochemical staining of mouse brain sections using Anti-P2Y12 Receptor Antibody (#APR-012), (red). The region shown in this picture is the entorhinal cortex in mouse brain. The P2Y12 positive fibers (arrows) run from the corpus callosum (triangles) into the deep layers of the entorhinal cortex. Sytox green is used as the counterstain.
    Immunocytochemistry
    • Rat astrocytes (1:50) (Carrasquero, L.M. et al. (2005) Purinergic Signal1, 153.).
    Indirect flow cytometry
    • Rat osteoclasts (8 μg/ml) (Alvarenga, E.C. et al. (2010) Bone 46, 355.).
    • The negative control antigen is not suitable for this application.
    References
    1. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
    2. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
    3. Abbracchio, M.P. et al. (2003) Trends. Pharmacol. Sci. 24, 52.
    4. Nicholas, R.A. (2001) Mol. Pharmacol. 60, 416.
    Scientific background

    The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1P2Y2P2Y4P2Y6P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.1-3 

    The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation.

    The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting.4 The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders.4

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:
    Expression of P2Y12 receptor in human eosinophils.A. Indirect flow cytometry of human eosinophils using Anti-P2Y12 Receptor Antibody (#APR-012). B. Immunocytochemical staining of human eosinophils using Anti-P2Y12 Receptor Antibody. P2Y12 immunostaining is shown in green. In both application, preincubating the antibody with the negative control antigen abolishes the detection of the receptor.Adapted from Muniz, V.S. et al. (2015) PLoS ONE 10, e0139805. with permission of PLoS.
    Last update: 12/03/2020

    Anti-P2Y12 Receptor Antibody (#APR-012) is a highly specific antibody directed against an intracellular epitope of the human P2RY12. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize P2RY12 from human, rat, and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. Mouse bone marrow lysate.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    2. Rat heart lysate (1:200).
      Giachini, F.R. et al. (2014) PLoS ONE 9, e91890.
    Immunoprecipitation citations
    1. Mouse bone marrow lysate.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    Immunocytochemistry citations
    1. Human eosinophils.
      Muniz, V.S. et al. (2015) PLoS ONE 10, e0139805.
    2. Human fibroblasts (1:100).
      Pinheiro, A.R. et al. (2013) J. Biol. Chem. 288, 27571.
    3. Rat astrocytes (1:50).
      Carrasquero, L.M. et al. (2005) Purinergic Signal. 1, 153.
    Immunofluorescence citations
    1. Human eosinophils.
      Muniz, V.S. et al. (2015) PLoS ONE 10, e0139805.
    Indirect flow cytometry citations
    1. Mouse bone marrow cells.
      Ribeiro-Filho, A.C. et al. (2016) BMC Pharmacol. Toxicol. 17, 29.
    2. Human eosinophils.
      Muniz, V.S. et al. (2015) PLoS ONE 10, e0139805.
    3. Rat osteoclasts (8 μg/ml).
      Alvarenga, E.C. et al. (2010) Bone 46, 355.
    More product citations
    1. Frobel, J. et al. (2013) Mol. Cell. Proteomics 12, 1272.
    2. Zerpa, H. et al. (2013) Eur. J. Pharmacol. 702, 242.
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