Name: Anti-P2Y2 Receptor (extracellular) Antibody
Brand: Alomone Labs
SKU: APR-102

Overview

Cat #: APR-102

Alternative Name P2Y purinoceptor 2, P2RY2, P2U1

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (CD)TINGTWEGDELGYK, corresponding to amino acid residues 11- 24 of rat P2RY2 (Accession P41232). Extracellular, C-terminus.

Accession (Uniprot) Number P41232

Gene ID 29597

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Rat, mouse – 14/15 amino acid residues identical; human – 13/15 amino acid residues identical.

RRID AB_2756769.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 0.8 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ifc, ih, lci, wb

May also work in: ip*

Western blot

Western blot analysis of mouse heart lysate (lanes 1 and 5), mouse brain lysate (lanes 2 and 6), rat brain lysate (lanes 3 and 7) and rat lung lysate (lanes 4 and 8):
1-4. Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102), (1:200).
5-8. Anti-P2Y2 Receptor (extracellular) Antibody, preincubated with P2Y2 Receptor (extracellular) Blocking Peptide (#BLP-PR102).

Immunohistochemistry

Expression of P2Y2 in mouse hippocampus
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. P2RY2 immunoreactivity (green) in the hippocampal dentate gyrus appears in blood vessels (horizontal arrows) and in neurons (vertical arrows). Cell nuclei are stained with DAPI (blue).

Indirect flow cytometry

Cell surface detection of P2RY2 in live intact mouse J774 macrophage cells:
^___ Cells.
^___ Cells + rabbit IgG isotype control + goat-anti-rabbit-FITC.
^___ Cells + Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102), (5 µg) + goat-anti-rabbit-FITC.

Live cell imaging / Immunocytochemistry

Mouse BV-2 microglia (Jiang, P. et al. (2017) PLoS One 12, e0183114 (2017).

References

Wang, L. et al. (2002) J. Cardiovas. Pharmacol. 40, 841.
Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809.
Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413.
Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci. 24, 52.
Di Virgilio, F. et al. (2001) Blood 97, 587.
Yerxa, B.R. et al. (2002) J. Pharmacol. Exp. Ther. 302, 871.

Scientific background

P2Y receptors belong to the G-protein coupled receptor superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiological responses, among them, cardiac function, platelet aggregation and SMC proliferation¹.

The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14^2-4.

The P2Y2 receptor is abundantly expressed in the apical membranes of several epithelial tissues including airway⁵. It has become a leading target for the treatment of cystic fibrosis. Activation of P2RY2 increases Cl^- efflux and mucociliary clearance, effects that might relieve symptoms of cystic fibrosis. P2Y2 agonist therapy is tested in an attempt to bypass the dysfunctioning CFTR channel. Several P2Y2 receptor agonists, especially INS37217, being evaluated as possible targets for the treatment of cystic fibrosis.⁶

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect live cell flow cytometry, live cell imaging, and immunohistochemistry applications. It has been designed to recognize P2RY2 from rat, mouse, and human samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 15/05/2023

Applications

Citations

Western blot citations

Mouse BV-2 microglia cell lysate (1:500).
Jiang, P. et al. (2017) PLoS One 12, e0183114 (2017).

Live cell imaging citations

Mouse BV-2 microglia (1:500).
Jiang, P. et al. (2017) PLoS One 12, e0183114 (2017).

Specifications

Scientific Background

Specific Control Product

P2Y2 Receptor (extracellular) Blocking Peptide (#BLP-PR102) is the original antigen used for immunization during Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102) generation. The blocking peptide binds and ‘blocks’ Anti-P2Y2 Receptor (extracellular) primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

P2Y2 Receptor (extracellular) Blocking Peptide (#BLP-PR102)

Shipping and Ordering Information

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Anti-P2Y2 Receptor (extracellular) Antibody