- Peptide (C)EWNPDLPHTAAKKEK corresponding to amino acid residues 301-315 of rat PACSIN1 (Accession Q9Z0W5). Intracellular.
- Western blot analysis of rat brain (lanes 1 and 3), mouse brain (lanes 2 and 4), (1:200) and human brain neuroblastoma SH-SY5Y cell line lysates (lanes 5 and 6), (1:600):1, 2, 5. Anti-PACSIN1 Antibody (#AIP-023).
3, 4, 6. Anti-PACSIN1 Antibody, preincubated with PACSIN1 Blocking Peptide (#BLP-IP023).
- Expression of PACSIN1 in rat hippocampus.Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-PACSIN1 Antibody (#AIP-023), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal CA1 region, showed PACSIN1 immunoreactivity (green) in some neurons (arrows) in the pyramidal layer (P). B. Pre-incubation of the antibody with PACSIN1 Blocking Peptide (BLP-IP023), suppressed staining. Cell nuclei are stained with DAPI (blue).
The protein kinase C and casein kinase substrate in neurons (PACSIN), also known as syndapin, is an F-BAR (elongated BAR) and SH3 (src homology-3) domain-containing protein that is capable of remodeling the plasma membrane and mediating protein-protein interactions.
Three different PACSIN variants are identified, which are differentially expressed: PACSIN I is the neuronal‐specific isoform, PACSIN II is ubiquitously expressed and PACSIN III is expressed mainly in skeletal muscles and heart1.
PACSINs interact with the large GTPase dynamin and several other proteins implicated in vesicle trafficking, PACSIN-dynamin complexes play an important role in vesicle fission at different donor membranes, including the plasma membrane (endocytosis) and Golgi membranes. In addition, PACSINs are implicated in later steps of vesicle cycling in neuronal and non-neuronal cells. PACSINs also engage in additional interactions with molecules involved in several signal transduction pathways, producing crosstalk at the interface between membrane trafficking and the cytoskeleton. Given the distinct expression patterns of the different PACSINs and their splice forms, these proteins could have isoform-specific functions2.
Species reactivity key:
Anti-PACSIN1 Antibody (#AIP-023) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize PACSIN1 from human, rat, and mouse samples.