Anti-PACSIN1 Antibody

Syndapin-1, Synaptic, Dynamin-associated protein I, Protein kinase C and casein kinase substrate in neurons protein 1
  • New
Cat #: AIP-023
Alternative Name Syndapin-1, Synaptic, Dynamin-associated protein I, Protein kinase C and casein kinase substrate in neurons protein 1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)EWNPDLPHTAAKKEK corresponding to amino acid residues 301-315 of rat PACSIN1 (Accession Q9Z0W5). Intracellular.
    • Anti-PACSIN1 Antibody
    Accession (Uniprot) Number Q9Z0W5
    Gene ID 29704
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 14/15 amino acid residues identical; human – 13/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ih*, ip*
    Western blot
    • Anti-PACSIN1 Antibody
      Western blot analysis of rat brain (lanes 1 and 3), mouse brain (lanes 2 and 4), (1:200) and human brain neuroblastoma SH-SY5Y cell line lysates (lanes 5 and 6), (1:600):
      1, 2, 5. Anti-PACSIN1 Antibody (#AIP-023).
      3, 4, 6. Anti-PACSIN1 Antibody, preincubated with the negative control antigen.
    References
    1. Widagdo, J. et al. (2016) Sci. Rep. 6, 31070.
    2. Kessels, M.M. and Qualmann, B. (2004) J. Cell Sci. 117, 3077.
    Scientific background

    The protein kinase C and casein kinase substrate in neurons (PACSIN), also known as syndapin, is an F-BAR (elongated BAR) and SH3 (src homology-3) domain-containing protein that is capable of remodeling the plasma membrane and mediating protein-protein interactions.

    Three different PACSIN variants are identified, which are differentially expressed: PACSIN I is the neuronal‐specific isoform, PACSIN II is ubiquitously expressed and PACSIN III is expressed mainly in skeletal muscles and heart1.

    PACSINs interact with the large GTPase dynamin and several other proteins implicated in vesicle trafficking, PACSIN-dynamin complexes play an important role in vesicle fission at different donor membranes, including the plasma membrane (endocytosis) and Golgi membranes. In addition, PACSINs are implicated in later steps of vesicle cycling in neuronal and non-neuronal cells. PACSINs also engage in additional interactions with molecules involved in several signal transduction pathways, producing crosstalk at the interface between membrane trafficking and the cytoskeleton. Given the distinct expression patterns of the different PACSINs and their splice forms, these proteins could have isoform-specific functions2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 22/01/2020

    Anti-PACSIN1 Antibody (#AIP-023) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize PACSIN1 from human, rat, and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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