Anti-Parathyroid Hormone 2 Receptor (extracellular)-ATTO-488 Antibody

PTH2 receptor
    Cat #: APR-052-AG
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    Immunogen
    Peptide CFLQPDINIGKQE, corresponding to amino acid residues 125-137 of rat Parathyroid Hormone 2 Receptor (Accession P70555). Extracellular, N-terminus.
    Accession (Uniprot) Number P70555
    Gene ID 81753
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 11/13 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody,#APR-052), and immunohistochemistry (labeled antibody).
    Applications: ih
    May also work in: ic, lci
    Immunohistochemistry
    Immuno-colocalization of Parathyroid Hormone 2 Receptor and Vesicular Glutamate Transporter 2 in mouse hippocampus
    Immunohistochemical staining of mouse hippocampal CA3 region frozen sections using Anti-Parathyroid Hormone 2 Receptor (extracellular)-ATTO-488 Antibody (#APR-052-AG), (1:60) and Anti-VGLUT2-ATTO-594 Antibody (#AGC-036-AR), (1:60). A. PTH2 receptor staining (green). B. VGLUT2 staining (red) in same section. C. Merge of A and B suggests extensive localization of PTH2R on glutamatergic neurons.
    References
    1. Bisello, A. et al. (2004) Mol. Endocrinol. 18, 1486.
    2. Usdin, T.B. et al. (1995) J. Biol. Chem270, 15455.
    3. Gensure, R.C. et al. (2005) Biochem. Biophys. Res. Commun. 328, 666.
    4. Hoare, S.R. et al. (1999) Endocrinology 140, 4419.
    5. Usdin, T.B. et al. (2002) Receptors Channels 8, 211.
    Scientific background

    PTH (parathyroid hormone) regulates Ca2+ and phosphate homeostasis through its action on specific receptors in kidney and bone. There are two known PTH receptors in humans, PTH receptor type 1 (PTH1 receptor) and PTH receptor type 2 (PTH2 receptor)1.

    The PTH2 receptor is a member of a class B subfamily of G-protein-coupled receptors that includes the receptors for the glucagon-GHRH-VIP family of peptides (glucagon, GLP-I, GIP, GHRHVIP, secretin, PACAP) and for calcitonin and CRF2. PTH2 receptor shares the same basic structure of the GPCR superfamily of proteins. A defining feature of the family B receptors is the relatively long extracellular N-terminal domain, which comprises about 150 amino acids and is important for ligand binding. The seven transmembrane domains are believed to be arranged in a circular or oval configuration, as seen in rhodopsin. The transmembrane domains are connected by three extracellular and three intracellular loops, and a C-terminal tail of about 130 amino acids extends intracellularly3.

    Northern blots of human messenger RNA (mRNA) show that the PTH2 receptor is most highly expressed in the central nervous system, preferentially in the hypothalamic regions and is also detected in the pancreas, testis, placenta, and lung4.

    Recent data support that PTH2 receptor is involved in hypothalamic releasing-factor secretion and pain5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 22/10/2018

    Anti-Parathyroid Hormone 2 Receptor (extracellular) Antibody (#APR-052) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize PTH2 receptor from human, rat and mouse samples.

    Anti-Parathyroid Hormone 2 Receptor (extracellular)-ATTO-488 Antibody (#APR-052-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Parathyroid Hormone 2 Receptor (extracellular)-ATTO-488 Antibody has been tested in immunohistochemistry applications and is especially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use
    Citations
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