Free shipping starts now, no minimum, no coupons required!

Anti-PMCA1 Antibody

Plasma membrane Ca2+ ATPase 1, ATP2B1

Cat #: ACP-005
Alternative Name Plasma membrane Ca2+ ATPase 1, ATP2B1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)SGVKNSLKEANHD, corresponding to amino acid residues 12-24 of human PMCA1 (Accession P20020). Intracellular, N-terminus.
Accession (Uniprot) Number P20020
Gene ID 490
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse - identical.
RRID AB_2756567.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity This antibody will recognize all known PMCA1 splice variants.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1,2. Anti-PMCA1 Antibody (#ACP-005), (1:200).
    3,4. Anti-PMCA1 Antibody, preincubated with PMCA1 Blocking Peptide (#BLP-CP005).
Immunohistochemistry
  • Expression of PMCA1 in mouse hippocampus
    Expression of PMCA1 in mouse hippocampus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-PMCA1 Antibody (#ACP-005), (1:300), followed by goat anti-rabbit-AlexaFluor-488 antibody. PMCA1 staining (green) appears in the stratum radiatum (SR) and oriens (OR) layers but not in the pyramidal (Pyr) layer of hippocampal CA1 region (arrows). Nuclei are labeled with DAPI (blue).
References
  1. Jensen, T.P. et al. (2004) Brain Res. Dev. Brain Res. 152, 129.
  2. Strehler, E.E. and Zacharias, D.A. (2001) Physiol. Rev. 81, 21.
  3. Talarico, E.F. et al. (2005) Mol. Vis. 11, 169.
  4. Jensen, T.P. et al. (2007) J. Physiol. 579, 85.
  5. Burette, A. and Weinberg, R.J. (2007) J. Comp. Neurol. 500, 1127.
Scientific background

The plasma membrane calcium ATPase (PMCA) is a transport protein in the plasma membrane of cells responsible for removing calcium (Ca2+) from the cell. This pump is vital for regulating the amount of Ca2+ within cells1.

PMCA and the sodium/calcium exchanger (NCX) are together the main regulators of intracellular Ca2+ concentrations2.

Since it transports Ca2+ into the extracellular space, PMCA is also an important regulator of the Ca2+ concentration in the extracellular space3.

The PMCA belongs to a family of P-type primary ion transport ATPases, and is expressed in a variety of tissues, including the brain4.

The pump is powered by the hydrolysis of adenosine triphosphate (ATP), with a stoichiometry of one Ca2+ ion removed for each molecule of ATP hydrolyzed. It binds to Ca2+ ions with a high affinity (a Km of 100 to 200 nM) but does not remove Ca2+ at a very fast rate. This is in contrast to the NCX, which has a low affinity and a high capacity. Thus, the PMCA is effective at binding Ca2+ even when its concentration within the cell is very low, and so is suited to maintaining Ca2+ at its normally very low levels. The NCX is better suited to removing large amounts of Ca2+ quickly, as it is needed in neurons after an action potential. Thus the activities of the two types of pump complement each other5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human Plasma membrane calcium-transporting ATPase 1. Anti-PMCA1 Antibody (#ACP-005) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PMCA1 from mouse, rat, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Shipping and Ordering information