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Anti-Prokineticin Receptor 2 (extracellular) Antibody

PROKR2, PKR2, G-protein coupled receptor 73-like 1, Gpr73l1, Gpr73b, G-protein coupled receptor I5E

Cat #: APR-042
Alternative Name PROKR2, PKR2, G-protein coupled receptor 73-like 1, Gpr73l1, Gpr73b, G-protein coupled receptor I5E
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
    Immunogen
    • Peptide (C)DQNGNTSFAPDLN, corresponding to amino acid residues 3-15 of rat PROKR2 (Accession Q8R415). Extracellular, N-terminus.
    Accession (Uniprot) Number Q8R415
    Gene ID 192649
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 11/13 amino acid residues identical; human - 9/13 amino acid residues identical.
    RRID AB_2039855.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: if, ifc, ih, lci, wb
    May also work in: ic*, ip*
    Western blot
    Immunohistochemistry
    • Expression of Prokineticin receptor 2 in rat DRG
      Expression of Prokineticin receptor 2 in rat DRG
      Immunohistochemical staining of rat dorsal root ganglion (DRG) frozen sections using Anti-Prokineticin Receptor 2 (extracellular) Antibody (#APR-042), (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). Staining is present in small, medium and large DRG neurons. Hoechst 33342 is used as the counterstain (blue).
    Indirect flow cytometry
    • Cell surface detection of Prokineticin receptor 2 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: 
      Cell surface detection of Prokineticin receptor 2 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: 
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-Prokineticin Receptor 2 (extracellular) Antibody (#APR-042), (2.5μg) + goat-anti-rabbit-FITC.
    References
    1. Zhou, Q.Y. (2006) Mol. Interv. 6, 330.
    2. Cheng, M.Y. et al. (2002) Nature 417, 405.
    3. Pitteloud, N. et al. (2007) Proc. Natl. Acad. Sci. U.S.A. 104, 17447.
    Scientific background

    The Prokineticins (PK1 and PK2) are a pair of cysteine-rich secreted peptides with broad physiological functions including gastrointestinal motility, angiogenesis, hematopoiesis and circadian rhythms regulation.

    The biological effects of PK1 and PK2 are mediated by two highly homologous receptors termed Prokineticin receptor 1 (PKR1) and Prokineticin receptor 2 (PKR2) that belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily. Both PK ligands activate the two PK receptors with similar potency.1

    PKR2 receptors couple to Gq/G11 proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization.

    The distribution of PKR2 is relatively restricted with high expression levels in the brain, spinal cord and dorsal root ganglions, in organs of the reproductive system and in endocrine tissues such as the thyroid, pituitary and adrenal glands.1

    The PK2 ligand has been shown to be involved in the regulation of circadian rhythms of physiological and behavioral processes in mammals, probably through signaling via PKR2 which is highly expressed in the suprachiasmatic nucleus (SCN), an area of the brain that controls circadian rhythm processes.2

    In addition, loss-of-function mutations in the PKR2 gene have been associated with Kallmann Syndrome, a condition characterized by idiopathic hypogonadotropic hypogonadism (IHH) in combination with anosmia, a compromised sense of smell.3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 02/08/2022

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the extracellular N-terminal domain of the rat Prokineticin receptor 2. The epitope is specific for PROKR2 and will not cross-react with the closely related PROKR1. Anti-Prokineticin Receptor 2 (extracellular) Antibody (#APR-042) can be used in western blot and immunohistochemical applications. It has been designed to recognize PROKR2 from rat, mouse, and human samples.

    For research purposes only, not for human use

    Applications

    Citations

    Citations
    KO validation citations
    1. Immunohistochemical staining of mouse DRG sections. Tested in PKR2-/- mice.
      Maftei, D. et al. (2020) Neuroscience 427, 16.
    Immunohistochemistry citations
    1. Mouse DRG sections. Tested in PKR2-/- mice.
      Maftei, D. et al. (2020) Neuroscience 427, 16.

    Specifications

    Scientific Background

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