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Anti-PrRP Receptor (GPR10) Antibody

Prolactin-releasing peptide receptor, PrRPR, Prolactin-releasing hormone receptor, PRLHR

Cat #: APR-054
Alternative Name Prolactin-releasing peptide receptor, PrRPR, Prolactin-releasing hormone receptor, PRLHR
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)RVSVKLRNRVVPGS, corresponding to amino acid residues 247-260 of rat PRLHR (Accession Q64121). 3rd intracellular loop.
Accession (Uniprot) Number Q64121
Gene ID 246075
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; human - 13/14 amino acid residues identical.
RRID AB_2341056.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), rat cerebellum (lanes 3 and 7) and PC-12 cells (lines 4 and 8):
    Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), rat cerebellum (lanes 3 and 7) and PC-12 cells (lines 4 and 8):
    1-4. Anti-PrRP Receptor (GPR10) Antibody (#APR-054), (1:1000).
    5-8. Anti-PrRP Receptor (GPR10) Antibody, preincubated with PrRP Receptor/GPR10 Blocking Peptide (#BLP-PR054).
References
  1. D’Ursi, A.M. et al. (2002) J. Med. Chem. 45, 5483.
  2. Lin, S.H. et al. (2001) Mol. Pharmacol. 60, 916.
  3. Roland, B.L. et al. (1999) Endocrinology 140, 5736.
  4. Gu W. et al. (2004) J. Mol. Neurosci. 22, 93.
  5. Bhattacharyya, S. et al. (2003) Diabetes 52, 1296.
Scientific background

Prolactin-releasing peptide (PrRP) a bioactive peptide, was originally identified as an endogenous ligand of the orphan human G-protein coupled receptor, seven transmembrane domain receptor, GPR10 (hGR3, also called PrRP receptor). Like the majority of orphan receptors, PrRP receptor shows a low level of sequence homology with known GPCRs. Notwithstanding, a certain degree of homology has been reported to members of the neuropeptide Y (NPY) family of receptors. Neuropeptide Y is a 36- residue C-terminally amidated polypeptide hormone and neurotransmitter with the presence of an α-helix involving the 20 C-terminal residues, whereas the N-terminus part seems more flexible and is influenced by the medium environment1. It has been recently demonstrated that the PrRP receptor may modulate neurotransmission specifically at glutamatergic synapses through the interaction with the same PDZ domain proteins that interact with AMPA receptors2.

Binding of PrRP to the receptor results in activation of extracellular signal-related kinase (ERK) in a mainly pertussis toxin sensitive manner, suggesting coupling to Gi/o proteins.

PrRP receptor is expressed in the central nervous system with highest levels in the pituitary. Expression has also been detected in the cerebellum, brainstem, hypothalamus, thalamus, nucleus tractus solitaries, adrenal medulla and spinal cord in rat3.

PrRP is implicated in the regulation of the hypothalamic–pituitary–adrenal axis, blood pressure and nociception4,5. Genetic studies in rodents indicate that lack of PrRP receptor leads to hyperphagia, obesity and dyslipidemia4. In humans, genetic variations in PrRP receptor are associated with lowered blood pressure5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/03/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Prolactin-releasing peptide receptor. Anti-PrRP Receptor (GPR10) Antibody (#APR-054) can be used in western blot analysis. It has been designed to recognize PrRPR from human, rat and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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