Overview
Type: Synthetic peptide
Form: Lyophilized powder
PrRP Receptor/GPR10 Blocking Peptide (#BLP-PR054) is the original antigen used for immunization during Anti-PrRP Receptor (GPR10) Antibody (#APR-054) generation. The blocking peptide binds and ‘blocks’ Anti-PrRP Receptor/GPR10 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.
Applications: wb, ihc
For research purposes only. not for human use
Applications
Demonstration of Pre-adsorption control
Western blot analysis of rat brain (lanes 1 and 5), mouse brain (lanes 2 and 6), rat cerebellum (lanes 3 and 7) and PC-12 cells (lines 4 and 8):1-4. Anti-PrRP Receptor (GPR10) Antibody (#APR-054), (1:1000).
5-8. Anti-PrRP Receptor (GPR10) Antibody, preincubated with PrRP Receptor/GPR10 Blocking Peptide (#BLP-PR054).
Properties
Sequence
- (C)RVSVKLRNRVVPGS, corresponding to amino acid residues 247-260 of rat PRLHR (Accession Q64121).
Accession (Uniprot) Number Q64121
Peptide Confirmation Confirmed by amino acid analysis and mass spectrometry.
Purity >70%
Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
Reconstitution 100 µl double distilled water (DDW).
Storage After Reconstitution -20°C.
Antigen Preadsorption Control 1 µg peptide per 1 µg antibody.
Standard Quality Control Of Each Lot Western blot analysis.
