Anti-Prostanoid EP4 Receptor Antibody

Prostaglandin E2 Receptor EP4 Subtype, PTGER4
  • New
Cat #: APR-066
Sizes: 25 µl, 50 µl, 0.2 ml
  • Lyophilized Powder
  • Antigen Incl.
  • QC Tested
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)RKTVLSKAIEKIK, corresponding to amino acid residues 336 - 348 of rat Prostanoid EP4 Receptor (Accession P43114). Intracellular, C-terminus.
    Accession (Uniprot) Number P43114
    Gene ID 84023
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
    Western blot
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse lung lysate (lanes 2 and 4):
    1, 2. Anti-Prostanoid EP4 Receptor Antibody (#APR-066), (1:200).
    3, 4. Anti-Prostanoid EP4 Receptor Antibody, preincubated with the control peptide antigen.
    Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):
    1, 2. Anti-Prostanoid EP4 Receptor Antibody (#APR-066), (1:400).
    3, 4. Anti-Prostanoid EP4 Receptor Antibody, preincubated with the control peptide antigen.
    References
    1. Konya, V. et al. (2013) Pharmacol. Ther. 138, 485.
    2. Markovič, T. et al. (2017) Drug Discov. Today 22, 57.
    3. Yokoyama, U. et al. (2013) Pharmacol. Rev. 65, 1010.
    Scientific background

    E-type prostanoid receptor 4 (EP4) is responsible for mediating the effects of prostaglandin E2 (PGE2), an endogenous molecule involved in several biological processes such as pain, fever, regulation of vascular tone, renal function, mucosal integrity, inflammation, angiogenesis and tumor growth. In turn, EP4 receptor is involved in anti-inflammatory, anti-thrombotic, vasoprotective effects, adverse tumor-promoting and pro-angiogenic roles1,2.

    EP4 mRNA expression is detected in the gastrointestinal tract, uterus, hematopoietic tissues and skin. High expression is also found in most immune cell types. The EP receptors activate various signaling pathways. EP4 is a Gs coupled receptor, which activates adenylate cyclase and activates intracellular cyclic adenylyl monophosphate (cAMP) production1,2.

    The EP receptors contain an aspartate in the second transmembrane domain, which is involved in receptor-ligand interaction and conserved cysteine residues in the second and third extracellular domains, which form a disulfide bonds critical for stabilizing the receptor and for ligand binding3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 15/04/2018

    Anti-Prostanoid EP4 Receptor Antibody (#APR-066) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Prostanoid EP4 Receptor from rat, mouse, and human samples.

    For research purposes only, not for human use