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Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody

Prostaglandin E2 receptor EP4 subtype, PGE2 receptor EP4 subtype, Prostanoid EP4 receptor

Cat #: APR-066
Alternative Name Prostaglandin E2 receptor EP4 subtype, PGE2 receptor EP4 subtype, Prostanoid EP4 receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RKTVLSKAIEKIK, corresponding to amino acid residues 336-348 of rat prostanoid EP4 receptor (Accession P43114). Intracellular, C-terminus.
Accession (Uniprot) Number P43114
Gene ID 84023
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2756762.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse lung lysate (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse lung lysate (lanes 2 and 4):
    1,2. Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody (#APR-066), (1:200).
    3,4. Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody, preincubated with Prostaglandin E Receptor EP4/PTGER4 Blocking Peptide (#BLP-PR066).
  • Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):
    Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human Jurkat T-cell leukemia cell line lysate (lanes 2 and 4):
    1,2. Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody (#APR-066), (1:400).
    3,4. Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody, preincubated with Prostaglandin E Receptor EP4/PTGER4 Blocking Peptide (#BLP-PR066).
Indirect flow cytometry
  • Human blood eosinophils (Durchschein, F. et al. (2019) Dig. Dis. Sci. 64, 2806.).
References
  1. Konya, V. et al. (2013) Pharmacol. Ther. 138, 485.
  2. Markovič, T. et al. (2017) Drug Discov. Today 22, 57.
  3. Yokoyama, U. et al. (2013) Pharmacol. Rev. 65, 1010.
Scientific background

E-type prostanoid receptor 4 (EP4) is responsible for mediating the effects of prostaglandin E2 (PGE2), an endogenous molecule involved in several biological processes such as pain, fever, regulation of vascular tone, renal function, mucosal integrity, inflammation, angiogenesis and tumor growth. In turn, EP4 receptor is involved in anti-inflammatory, anti-thrombotic, vasoprotective effects, adverse tumor-promoting and pro-angiogenic roles1,2.

EP4 mRNA expression is detected in the gastrointestinal tract, uterus, hematopoietic tissues and skin. High expression is also found in most immune cell types. The EP receptors activate various signaling pathways. EP4 is a Gs coupled receptor, which activates adenylate cyclase and activates intracellular cyclic adenylyl monophosphate (cAMP) production1,2.

The EP receptors contain an aspartate in the second transmembrane domain, which is involved in receptor-ligand interaction and conserved cysteine residues in the second and third extracellular domains, which form a disulfide bonds critical for stabilizing the receptor and for ligand binding3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 25/07/2021

Anti-Prostaglandin E Receptor EP4 (PTGER4) Antibody (#APR-066) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize EP4 receptor from rat, mouse, and human samples.

For research purposes only, not for human use
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