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Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody

Prorenin Receptor, Renin/prorenin receptor, PRR, (pro)renin receptor, ATPase H+ Transporting Accessory Protein 2, ATP6IP2
Cat #: AAR-014
Alternative Name Prorenin Receptor, Renin/prorenin receptor, PRR, (pro)renin receptor, ATPase H+ Transporting Accessory Protein 2, ATP6IP2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RHKHLAKDHSPDLYS, corresponding to amino acid residues 200 - 214 of mouse Renin Receptor (Accession Q9CYN9). Extracellular, N-terminus.
Accession (Uniprot) Number Q9CYN9
Gene ID 70495
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human – identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Specificity This antibody will recognize both the membrane form of the Renin Receptor and the soluble cleaved form (sPRR).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat heart membranes (lanes 1 and 4), mouse kidney membranes (lanes 2 and 5) and rat brain membranes (lanes 3 and 6):
    Western blot analysis of rat heart membranes (lanes 1 and 4), mouse kidney membranes (lanes 2 and 5) and rat brain membranes (lanes 3 and 6):
    1-3. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (1:200).
    4-6. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody, preincubated with Renin Receptor/ATP6AP2 (extracellular) Blocking Peptide (#BLP-AR014).
  • Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and mouse BV-2 microglia cell line lysate (lanes 2 and 4):
    Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and mouse BV-2 microglia cell line lysate (lanes 2 and 4):
    1, 2. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (1:200).
    3, 4. Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody, preincubated with Renin Receptor/ATP6AP2 (extracellular) Blocking Peptide (#BLP-AR014).
Indirect flow cytometry
  • Cell surface detection of Renin Receptor in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of Renin Receptor in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014), (2.5 µg) + goat-anti-rabbit-FITC.
References
  1. Ichihara, A. and Yatabe, M.S. (2019) Nat. Rev. Nephrol. 15, 693.
Scientific background

The (pro) Renin receptor, (p)RR, encoded by ATP6AP2 gene in humans, is a single pass transmembrane protein that binds both Renin and its inactive precursor pro-renin. ATP6AP2 is highly conserved among species, and (Pro) Renin Receptor orthologues are expressed in species from mammals to C. elegans and Drospohila melanogaster1.

Binding of Pro-renin to (p)RR receptor facilitates its truncation and generation of Renin. Renin is an aspartyl protease that cleaves angiotensinogen into angiotensin I, the rate-limiting reaction in the cascade generating angiotensin, a peptide hormone that is involved in blood pressure regulation. Renin binding by (P)RR affects important biological systems and pathways that are involved in cell cycle regulation, autophagy, acid-base balance, energy metabolism, embryonic development, T cell homeostasis, water balance, blood pressure regulation, cardiac remodeling and maintenance of podocyte structure1.

In addition, (P)RR has been reported to contribute to the pathogenesis of diseases such as fibrosis, hypertension, pre-eclampsia, diabetic microangiopathy, acute kidney injury, cardiovascular disease, cancer and obesity1.

The receptor is widely distributed in organs throughout the body, including the kidneys, heart, brain, eyes, placenta, and the immune system1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 09/11/2020

Anti-Renin Receptor/ATP6AP2 (extracellular) Antibody (#AAR-014) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize ATP6AP2 from rat, mouse, and human samples. This antibody will recognize both the membrane form of the Renin Receptor and the soluble cleaved form (sPRR).

For research purposes only, not for human use
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