- Peptide (C)RAKRGRSAESLHMTSE, corresponding to amino acid residues 1039-1054 of rat RIM1 (Accession Q9JIR4). Intracellular.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-RIM1 Antibody (#AIP-014), (1:200).
3,4. Anti-RIM1 Antibody, preincubated with RIM1 Blocking Peptide (#BLP-IP014).
RIM1 is a member of the RIM superfamily of proteins (Rim1-4). These genes express seven known RIM isoforms: RIM1α, 1β; RIM2α, 2β, 2γ; and RIM3γ and RIM4γ which serve as scaffolding proteins in presynaptic nerve terminals by interacting with several other protein components localized at the active zone, including Munc13, ELKS (or CAST), liprins and voltage-gated Ca2+ channels (VGCCs). RIM1 directly binds to the C-terminal regions of the α1 subunit of N- and P/Q-type calcium channels, and can also interact with the β auxiliary subunit of P/Q-type calcium channels1-3.
RIM proteins were found to be required for normal Ca2+ triggering of exocytosis. This interaction with Ca2+ channel significantly slows channel inactivation resulting in increased Ca2+ influx during action potentials.
Deficiency of RIM1 decreases presynaptic Ca2+ channel density. RIM proteins are involved in neurotransmitter release and contribute to long- and short-term synaptic plasticity.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat RIM1. Anti-RIM1 Antibody (#AIP-014) can be used in western blot analysis. It has been designed to recognize RIM1 from mouse, rat and human samples.