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2. Anti-CACNA1B (CaV2.2) Antibody, preincubated with the control antigen.
- Tsunemi, T. et al. (2002) J. Biol. Chem. 277, 7214.
- Beuckmann, C.T. et al. (2003) J. Neurosci. 23, 6793.
- Scheuber, A. et al. (2004) J. Neurosci. 24, 10402.
- Dascal, N. (2001) Trends Endocrinol. Metab. 12, 391.
- McCleskey, E.W. et al. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 4327.
- Feng, Z.P. et al. (2001) J. Biol. Chem. 276, 15728.
Voltage-dependent Ca2+ channels (CaV channels) are pivotal players in many physiological roles such as secretion, contraction, migration and excitation.1
The voltage-dependent Ca2+ channels are composed of several subunits; α1, β, α2δ and γ. CaV channels were originally divided into six physiological types: L-, N-, P-, Q-, R-, and T-type.
The CaV2.2 (formally named α1B) composes the α1 poreforming subunit for the N-type Ca2+ channel family. They are involved in neurotransmitter release from central neurons, including glutamate, γ-aminobutyric acid, acetylcholine, dopamine and noradrenaline.2
The CaV2.2 is expressed preferentially in the central nervous system, where along with CaV2.1, it is responsible for pre-synaptic Ca2+ influx and neurotransmitter release.1,3
The CaV2.2 channel is negatively regulated by many different GPCRs. There are two ways that this is done: either by directly binding Gβγ to the channel or by an indirect mechanism involving second messenger and channel phosphorylation.4
ω-Conotoxin GVIA (#C-300) is a specific blocker of CaV2.2 Ca2+ channels. It specifically blocks N-type CaV channels by binding to the CaV2.2 α1 subunit (α1B) and its action is only partially reversible.5,6
Species reactivity key:
Anti-CACNA1B (CaV2.2) Antibody (#ACC-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize CaV2.2 from mouse, rat, and human samples.
- Transfected tsA201 cells (1:200).
Marangoudakis, S. et al. (2012) J. Neurosci. 32, 10365.
- Mouse kidney sections.
Ohno, S. et al. (2016) Sci. Rep. 6, 27192.
- Rat retinal sections (1:1000).
Sargoy, A. et al. (2014) PLoS ONE 9, e84507.
- Rat intracardiac ganglia and stellate ganglia neurons.
Tu, H. et al. (2014) Am. J. Physiol. 306, C132.
- Rat retinal sections (1:1000-1:1500).
Liu, X. et al. (2013) J. Physiol. 591, 3309.
- Rat adipose-derived stromal cells and rat bone marrow stromal cells.
Forostyak, O. et al. (2016) Stem Cell Res. 16, 622.
- Rat stellate neurons (1:200).
Larsen, H.E. et al. (2016) J. Neurosci. 36, 8562.
- Rat primary submucosa cells (1:100).
Rehn, M. et al. (2013) Cell Tissue Res. 353, 355.
- NCI-H295R human adrenocortical cell line (H295R) (1:100).
Aritomi, S. et al. (2011) Hypertens. Res. 34, 193.
- Mishima, K. et al. (2013) Am. J. Physiol. 304, F665.
- Lv, P. et al. (2012) J. Neurosci. 32, 16314.
- Shoham, S. et al. (2001) Biol. Psychiatry 49, 876.
- Schiff, M.L. et al. (2000) Nature 408, 723.
- Singer-Lahat, D. et al. (2000) Eur. J. Physiol. 440, 627.
- Bae, I.H. et al. (1999) Korean J. Biol. Sci. 3, 53.
- Barbosa, J. et al. (1999) J. Neurochem. 73, 1881.
- Lopez, I. et al. (1999) Neuroscience 92, 773.
- Macleod, G.T. et al. (1999) J. Neurophysiol. 82, 1133.
- Serrano, C.J. et al. (1999) FEBS Letters 462, 171.
- Anti-CACNA1A (CaV2.1) Antibody (#ACC-001)
- Anti-CaV2.3 (CACNA1E) Antibody (#ACC-006)
- Anti-CACNA2D2 (CaVα2δ2) (extracellular) Antibody (#ACC-102)
- Anti-CaVα2δ3 (extracellular) Antibody (#ACC-103)
- Anti-CaVα2δ4 (extracellular) Antibody (#ACC-104)
- Anti-CASK Antibody (#AIP-018)
- Anti-RIM1 Antibody (#AIP-014)
- Anti-Sigma-1 Receptor Antibody (#AIP-006)
- Blockers/Antagonists: peptides/peptide toxinsBlockers/Antagonists: small moleculesActivators/Agonists: small molecules