Anti-Ryanodine Receptor 3 Antibody

RyR3, Brain-type ryanodine receptor
    Cat #: ARR-003
    Alternative Name RyR3, Brain-type ryanodine receptor
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide CHIAHVNPAAEEDLK, corresponding to amino acid residues 935-949 of human RyR3 (Accession Q15413). Intracellular, N-terminus.
    • Anti-Ryanodine Receptor 3 Antibody
    Accession (Uniprot) Number Q15413
    Gene ID 6263
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - identical.
    RRID AB_2040186.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.75 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
    Western blot
    • Anti-Ryanodine Receptor 3 Antibody
      Western blot analysis of rat brain membranes:
      1. Anti-Ryanodine Receptor 3 Antibody (#ARR-003), (1:200).
      2. Anti-Ryanodine Receptor 3 Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-Ryanodine Receptor 3 Antibody
      Expression of RyR3 in rat cortex
      Immunohistochemical staining of rat brain frozen sections with Anti-Ryanodine Receptor 3 Antibody (#ARR-003), (red), (1:100). RyR3 is widely expressed in neurons of the cortex. Hoechst 33342 is used as the counterstain.
    Immunocytochemistry
    • Human ESC NPs (1:100) (Forostyak, O. et al. (2013) Stem Cells Dev. 22, 1506.).
    References
    1. Chakrabarti, R. and Chakrabarti, R. (2006) J. Cell. Biochem. 99, 1503.
    2. Eisner, D.A. et al. (2005) Exp. Physiol. 90, 3.
    3. Hamilton, S.L. (2005) Cell Calcium 38, 253
    4. Fill, M. and Copello, J.A. (2002) Physiol. Rev. 82, 893.
    Scientific background

    It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2  The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.

    The ER and SR contain two Ca2+ release channel families, the inositol trisphosphate receptors (IP3Rs) and the ryanodine receptors (RyRs).3

    The ryanodine receptor family consists of three different isoforms: the skeletal muscle isoform, ryanodine receptor type 1 (RyR1); the cardiac muscle isoform, ryanodine receptor type 2 (RyR2) and the brain isoform, ryanodine receptor type 3 (RyR3).3

    The ryanodine receptors are homotetrameric proteins, which upon assembly create a Ca2+ conducting channel. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of ryanodine (a poisonous alkaloid found in the South American plant Ryania speciosa) to the ryanodine receptor causes two major changes in the channel: a reduction in single-channel conductance and a marked increase in the open state probability, leading to an overall increase/decrease in the Ca2+ release capability of the channel.

    RyR3 is the most widely expressed RyR channel which is predominantly expressed in smooth muscle tissues and certain regions of the brain.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-Ryanodine Receptor 3 Antibody
    Expression of RyR3 in rat PASMCs.Immunocytochemical staining of rat pulmonary arterial smooth muscle cells (PASMCs) using Anti-Ryanodine Receptor 3 Antibody (#ARR-003), (red). Cav-1 is stained in green.Adapted from Gilbert, G. et al. (2016) Am. J. Physiol. 310, L1078. with permission of The American Physiological Society.

    Last update: 19/03/2020

    Anti-Ryanodine Receptor 3 Antibody (#ARR-003) is a highly specific antibody directed against an epitope of human RyR3. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize RyR3 from human, rat, and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. Mouse smooth muscle cell lysate (1:200).
      Lee, M.Y. et al. (2015) PLoS ONE 10, e0133751.
    Immunocytochemistry citations
    1. Rat adipose-derived stromal cells and rat bone marrow stromal cells.
      Forostyak, O. et al. (2016) Stem Cell Res. 16, 622.
    2. Human ESC NPs (1:100).
      Forostyak, O. et al. (2013) Stem Cells Dev. 22, 1506.
    More product citations
    1. Yoshida, S. et al. (2010) Neurosci. Res. 68, 322.
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