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Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody

SEMAB

Cat #: ASR-065
Alternative Name SEMAB
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KELNKETSRWTTYRG, corresponding to amino acid residues 355 - 369 of human Semaphorin 4A (Accession Q9H3S1). Extracellular, N-terminus.
Accession (Uniprot) Number Q9H3S1
Gene ID 64218
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat – identical.
Purity Affinity purified on immobilized antigen.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis
Applications: ifc, ih, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
    1-2. Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody (#ASR-065), (1:400).
    3-4. Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody, preincubated with Semaphorin 4A (SEMA4A) (extracellular) Blocking Peptide (BLP-SR065).
  • Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 4):
    Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human HL-60 promyelocytic leukemia cell line lysate (lanes 2 and 4):
    1-2. Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody (#ASR-065), (1:400).
    3-4. Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody, preincubated with Semaphorin 4A (SEMA4A) (extracellular) Blocking Peptide (BLP-SR065).
Immunohistochemistry
  • Expression of Semaphorin 4A in rat hypothalamus
    Expression of Semaphorin 4A in rat hypothalamus
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody (#ASR-065), (1:300), followed by goat anti-rabbit-Alexa-488. A. Semaphorin 4A immunoreactivity (green) appears in the ventricular wall (horizontal arrows) and in tanycyte processes (vertical arrows).  B. Pre-incubation of the antibody with Semaphorin 4A (SEMA4A) (extracellular) Blocking Peptide (BLP-SR065), suppressed staining. Cell nuclei are stained with DAPI (blue). 3V = 3rd cerebral ventricle,
    VMH = ventromedial hypothalamus.
Indirect flow cytometry
  • Cell surface detection of Semaphorin 4A by indirect flow cytometry in live intact mouse J774 monocyte cells:
    Cell surface detection of Semaphorin 4A by indirect flow cytometry in live intact mouse J774 monocyte cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody (#ASR-065), (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Lyer and Chapoval (2018), Int J Mol Sci, 20, 124.
  2. Kumanogoh and Kikutani  (2003), J. Cell. Sci, 116, 363.
  3. Nkyimbeng-Takwi and Chapoval, (2011) Immunol Res., 50, 10.
  4. Luchino et al (2013), Cancer Cell, 24, 673.
Scientific background

Neuroimmune Semaphorin 4A (Sema4A) is a member of Semaphorin family of transmembrane and secreted proteins.

The Semaphorin protein family members regulate the functional activity of axons in the nervous system, and Sema4A is an important regulator of neuronal and immune functions 1,2.  In accordance to that, it was shown to be preferentially expressed on dendritic cells and B cells in the immune system 3.

Sema4A consists of an NH2-terminal signal peptide followed by a Sema domain, an Ig domain of the C2 type, a hydrophobic transmembrane region, and a cytoplasmic tail 2, it has seven currently known receptors through which it mediates a complex system of intracellular and extracellular signals that regulate different physiological and pathological tissue processes 1

In the nervous system, Sema4A serves as an axon guidance molecule and in the immune system it regulates immune cell activation and function, instructing the fine tuning of the immune response 1.

Recent studies have shown a dysregulation of Sema4A expression in several types of cancer such as hepatocellular carcinoma, colorectal, and breast cancers 1,4. The function of Sema4A in angiogenesis and cancer is not defined but it is shown to be related to the process of angiogenesis. Understanding the role of Sema4A in pathologic processes underlining tumorigenesis and tumor metastasis may guide the development of improved therapeutic treatments for cancer 1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 02/03/2021

Anti-Semaphorin 4A (SEMA4A) (extracellular) Antibody (#ASR-065) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize Semaphorin 4A from mouse, rat and human samples.

For research purposes only, not for human use
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