- Peptide CTPNKPSRTSMSK, corresponding to amino acid residues 498-510 of rat SERCA2 (Accession P11507). 2nd cytoplasmic loop.
- Western blot analysis of rat heart membranes (lanes 1 and 5), mouse heart membranes (lanes 2 and 6), rat PC12 pheochromocytoma cell line lysate (lanes 3 and 7) and human Jurkat T cell leukemia cell line lysate (lanes 4 and 8):1-4. Anti-SERCA2 Antibody (#ACP-012), (1:200).
5-8. Anti-SERCA2 Antibody, preincubated with SERCA2 Blocking Peptide (#BLP-CP012).
- Expression of SERCA2 in mouse C2C12 cellsImmunocytochemical staining of fixed and permeabilized mouse myoblast C2C12 cells. A. Cells were stained using Anti-SERCA2 Antibody (#ACP-012), (1:300) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Merged image of SERCA2 labeling with a live image of the cells. Cell nuclei were visualized using Hoechst 33342 (blue).
Three Ca2+ ATPases have been described in mammalian cells. They are located in the plasma membrane, endoplasmic reticulum or the Golgi apparatus. SERCA pumps are located in both the endoplasmic reticulum and in the Golgi membranes. They are known to transport two Ca2+ molecules per hydrolysis of one ATP1. Their structure includes ten transmembrane domains and their main role is to remove cytoplasmic Ca2+ ions in order to promote muscle relaxation1.
In mammals three genes encode three SERCA pumps. Each transcript undergoes tissue-dependent alternative splicing. SERCA1a and 1b are expressed in adult and neonatal skeletal muscle respectively. SERCA2a is also expressed in skeletal muscle, while SERCA2b is ubiquitously expressed. SERCA3 is expressed in a limited number of non-muscle cells2. Although all SERCAs are regulated, SERCA2b undergoes extensive regulation at the protein level, such as protein-protein interaction, phosphorylation and glycosylation1.
SERCA2 catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization3,4.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat SERCA2. Anti-SERCA2 Antibody (#ACP-012) can be used in western blot and immunocytochemistry applications and was designed to recognize SERCA2 from rat, mouse and human samples.