Anti-Serotonin Transporter (SERT) (extracellular)-FITC Antibody

Sodium-dependent serotonin transporter, 5-HTT, 5-HTTLPR, SLC6A4
  • New
Cat #: AMT-004-F
Sizes: 50 µl
  • Lyophilized Powder
  • Antigen Incl.
  • FITC
  • QC Tested
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)EMRNEDVSEVAKD, corresponding to amino acids residues 388-400 of rat Serotonin Transporter (Accession P31652).  4th extracellular loop.
    Accession (Uniprot) Number P31652
    Gene ID 25553
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #AMT-004), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic*, lci*
    Direct flow cytometry
    Direct flow cytometry of live intact human MEG-01 megakaryoblastic leukemia cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Serotonin Transporter (SERT) (extracellular)-FITC Antibody (#AMT-004-F), (5 µg).
    Direct flow cytometry of live intact mouse P815 mastocytoma cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Serotonin Transporter (SERT) (extracellular)-FITC Antibody (#AMT-004-F), (5 µg).
    References
    1. Haenisch, B. and Bönisch, H. (2011) Pharmacol. Ther. 129, 352.
    2. Ramamoorthy, S. et al. (2010) Pharmacol. Ther. 129, 220.
    3. Kim, D.K. et al. (2005) Neuropharmacology 49, 798.
    4. Mathews, T.A. et al. (2004) J. Neurosci. Meth. 140, 169.
    5. Gainetdinov, R.R. and Caron, M.G. (2003) Annu. Rev. Pharmacol. Toxicol. 43, 261.
    6. Greengard, P. (2001) Science. 294, 1024.
    7. Miller, G.W. et al. (1999) Trends. Pharmacol. Sci. 20, 424.
    8. Amara, S.G. and Kuhar, M.J. (1993) Annu. Rev. Neurosci. 16, 73.
    9. Carlsson, A. (1987) Annu. Rev. Neurosci. 10, 19.
    Scientific background

    Many physiological, endocrine and behavioral functions are determined and regulated by monoamine signaling1,2. Many brain disorders such as depression, drug abuse, schizophrenia, attention deficit hyperactivity disorder (ADHD) are caused by the malfunction of monoaminergic transmission1-3. The intensity of monoaminergic signaling is determined by the availability of the monoamine, which is in turn determined in part by its uptake from the extracellular milieu via monoamine transporters. These transporters include DAT, SERT, and NET, responsible for uptaking dopamine, serotonin (5-HT) and noradrenaline respectively, and recycling them back for release3-5.

    DAT, SERT and NET are members of the Na+/Cl- dependent membrane transporter family which also includes other members. These transporters consist of 12 transmembrane domains and intracellular N- and C-termini. Like its counterparts, SERT’s intracellular N- and C-terminal domains are also subject to phosphorylation and protein-protein interactions important for modulating its activity and localization6.

    SERTs are evidently expressed in serotonergic neurons, and are also expressed in various peripheral tissues including specialized cells of the gut, placenta, lung, blood lymphocytes and platelets6.

    SERT knockout mice are viable and as a consequence exhibit increased extracellular levels of 5-HT and significantly decreased levels of brain tissue 5-HT due to deficient recycling and re-accumulation of serotonin by SERT7,8. Phenotypes displayed by SERT knockout mice include stress sensitivity, obesity and various behavioral changes9.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 25/04/2018

    Anti-Serotonin Transporter (SERT) (extracellular) Antibody (#AMT-004) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and indirect flow cytometry, and live cell imaging applications. It has been designed to recognize SERT from human, mouse, and rat samples.

    Anti-Serotonin Transporter (SERT) (extracellular)-FITC Antibody (#AMT-004-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

    For research purposes only, not for human use