Anti-SLC28A3/CNT3 (extracellular) Antibody

Solute Carrier Family 28 Member 3, Concentrative Na+-Nucleoside Cotransporter 3
  • New
Cat #: ANT-063
Alternative Name Solute Carrier Family 28 Member 3, Concentrative Na+-Nucleoside Cotransporter 3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide (C)KITLKNAMKMENGDSR, corresponding to amino acid residues 432 - 447 of rat SLC28A3/CNT3 (Accession Q8VIH3). 5th extracellular loop.
    • Anti-SLC28A3/CNT3 (extracellular) Antibody
    Accession (Uniprot) Number Q8VIH3
    Gene ID 140944
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 15/16 amino acid residues identical; human – 14/16 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
    Western blot
    • Anti-SLC28A3/CNT3 (extracellular) Antibody
      Western blot analysis of mouse kidney membranes (lanes 1 and 5), rat pancreas lysates (lanes 2 and 6), mouse brain membranes (lanes 3 and 7) and rat brain lysate (lanes 4 and 8):
      1-4. Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063), (1:400).
      5-8. Anti-SLC28A3/CNT3 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-SLC28A3/CNT3 (extracellular) Antibody
      Western blot analysis of human HL-60 myelocytic leukemia cell line lysate (lanes 1 and 5), human K562 chronic myelogenous leukemia cell line lysate (lanes 2 and 6), human MCF-7 breast adenocarcinoma cell line lysate (lanes 3 and 7) and human PANC-1 pancreatic carcinoma cell line lysate (lanes 4 and 8):
      1-4. Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063), (1:500).
      5-8. Anti-SLC28A3/CNT3 (extracellular) Antibody, preincubated with the negative control antigen.
    Indirect flow cytometry
    • Anti-SLC28A3/CNT3 (extracellular) Antibody
      Cell surface detection of SLC28A3/CNT3 in live intact human HL-60 promyelocytic leukemia cells:
      ___ Cells.
      ___ Cells + goat-anti-rabbit-FITC.
      ___ Cells + Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063), (5 µg) + goat-anti-rabbit-FITC.
    1. Badagnani, I. et al. (2005) Pharmacogenomics J. 5, 157.
    2. Stecula, A. et al. (2017) Biochemistry 56, 3475.
    3. Damaraju, S. et al. (2005) Pharmacogenet. Genomics 15, 173.
    4. Gray, J.H. et al. (2004) Pflugers Arch. 447, 728.
    Scientific background

    Solute carrier family 28 member 3 (SLC28A3, CNT3) is a sodium-dependent, concentrative pyrimidine- and purine-selective transporter. SLC28A3 plays an important role in mediating the cellular entry of a broad array of physiological nucleosides and synthetic anticancer nucleoside analog drugs, in a process that is coupled to the sodium cellular gradient1.

    CNT3 consists of 13 putative trans-membrane domains and it forms a homo-trimer which is important for its activity as a transporter2,3.

    SLC28 members are crucial determinants in the response to a variety of anticancer and antiviral nucleoside analogs, as they modulate the entry of these analogs into target tissues. Within this large group of solute carriers, SLC28A is a sub-group which consists of SLC28A1, SLC28A2, and SLC28A3. Of the three members of this sub-family, SLC28A3 has the broadest selectivity and the widest expression profile4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 23/07/2020

    Anti-SLC28A3/CNT3 (extracellular) Antibody (#ANT-063) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize CNT3 from human, rat, and mouse samples.

    For research purposes only, not for human use
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