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Anti-SNAP-25 Antibody

Synaptosomal-associated protein 25 kD

Cat #: ANR-001
Alternative Name Synaptosomal-associated protein 25 kD
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • GST fusion protein with the sequence MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARV VDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG, corresponding to the full-length of rat SNAP-25 (Accession P60881). Cytoplasmic.
Accession (Uniprot) Number P60881
Gene ID 25012
Peptide confirmation Confirmed by DNA sequence and SDS-PAGE.
Homology Human, mouse - identical.
RRID AB_2040196.
Purity The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat brain membranes:
    Western blot analysis of rat brain membranes:
    1. Anti-SNAP-25 Antibody (#ANR-001), (1:1000).
    2. Anti-SNAP-25 Antibody, preincubated with SNAP-25 Blocking Peptide (BLP-NR001).
Immunohistochemistry
  • Rat brain sections.
References
  1. Jahn, R. and Scheller, R.H. (2006) Nat. Rev. Mol. Cell Biol. 7, 631.
  2. Südhof, T.C. and Rothman, J.E. (2009) Science 323, 474.
  3. Schiavo, G. et al. (2000) Physiol. Rev. 80, 717.
Scientific background

SNAP-25 (synaptosomal-associated 25kD protein) is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60–70 amino acids that are arranged in heptad repeats1,2.

SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.

SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.

SNAP-25, unlike most SNAREs, lacks a membrane-spanning region, but is attached to the cytoplasmic side of the plasma membrane by posttranslational modifications such as palmitoylation or farnesylation1,2.

SNAP-25 has been extensively studied for its role on neuronal and neuroendocrine cell exocytosis where it functions as one of the plasma membrane protein t-SNARE, which together with the vesicular v-SNARE protein VAMP and another t-SNARE such as Syntaxin 1, forms a trimeric, four-helical complex, which drives fusion of the two opposing bilayers1,2.

SNAP-25 is the target of several botulinum neurotoxin (BoNT) types:  type A, C and E. The neurotoxins cause specific proteolytic degradation of the SNAP-25 protein, which in turn causes SNARE complex disruption and inhibition of neurotransmitter release3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Alomone Labs is pleased to offer a highly specific antibody directed against rat SNAP-25. Anti-SNAP-25 Antibody (#ANR-001) can be used in western blot and immunohistochemical applications. It has been designed to recognize SNAP-25 from rat, mouse and human samples.

For research purposes only, not for human use

Applications

Citations

Citations
Western blot citations
  1. Mouse brain lysate.
    Chaboub, L.S. et al. (2016) J. Neurosci. 36, 11904.
  2. Zebrafish embryos.
    Wei, C. et al. (2013) PLoS ONE 8, e57080.
More product citations
  1. Xie, Z. et al. (2013) J. Neurophysiol. 109, 758.
  2. Fili, O. et. al. (2001) J.Neurosci. 21, 1964.
  3. Gerona, R.R. et al. (2000) J. Biol. Chem. 275, 6328.
  4. Kolk, S.M. et al. (2000) J. Neuroendocrinol. 12, 694.
  5. Morel, N. et al. (1998) J. Physiol. 92, 389.
  6. O'Connor, V. et al. (1997) Proc. Natl. Acad. Sci. U.S.A. 94, 12186.

Specifications

Scientific Background

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