- GST fusion protein with the sequence MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARV VDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG, corresponding to the full-length of rat SNAP-25 (Accession P60881). Cytoplasmic.
- Western blot analysis of rat brain membranes:1. Anti-SNAP-25 Antibody (#ANR-001), (1:1000).
2. Anti-SNAP-25 Antibody, preincubated with SNAP-25 Blocking Peptide (BLP-NR001).
- Rat brain sections.
SNAP-25 (synaptosomal-associated 25kD protein) is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60–70 amino acids that are arranged in heptad repeats1,2.
SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.
SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.
SNAP-25, unlike most SNAREs, lacks a membrane-spanning region, but is attached to the cytoplasmic side of the plasma membrane by posttranslational modifications such as palmitoylation or farnesylation1,2.
SNAP-25 has been extensively studied for its role on neuronal and neuroendocrine cell exocytosis where it functions as one of the plasma membrane protein t-SNARE, which together with the vesicular v-SNARE protein VAMP and another t-SNARE such as Syntaxin 1, forms a trimeric, four-helical complex, which drives fusion of the two opposing bilayers1,2.
SNAP-25 is the target of several botulinum neurotoxin (BoNT) types: type A, C and E. The neurotoxins cause specific proteolytic degradation of the SNAP-25 protein, which in turn causes SNARE complex disruption and inhibition of neurotransmitter release3.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against rat SNAP-25. Anti-SNAP-25 Antibody (#ANR-001) can be used in western blot and immunohistochemical applications. It has been designed to recognize SNAP-25 from rat, mouse and human samples.
- Xie, Z. et al. (2013) J. Neurophysiol. 109, 758.
- Fili, O. et. al. (2001) J.Neurosci. 21, 1964.
- Gerona, R.R. et al. (2000) J. Biol. Chem. 275, 6328.
- Kolk, S.M. et al. (2000) J. Neuroendocrinol. 12, 694.
- Morel, N. et al. (1998) J. Physiol. 92, 389.
- O'Connor, V. et al. (1997) Proc. Natl. Acad. Sci. U.S.A. 94, 12186.