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Anti-NALCN/VGCNL1 (extracellular) Antibody

Sodium leak channel non-selective protein, Voltage gated channel-like protein 1, Four domain-type voltage-gated ion channel alpha-1 subunit, CanIon, Rb21-channel

Cat #: ASC-022
Alternative Name Sodium leak channel non-selective protein, Voltage gated channel-like protein 1, Four domain-type voltage-gated ion channel alpha-1 subunit, CanIon, Rb21-channel
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)SMMFESPFRRVMH, corresponding to amino acid residues 902-914 of rat NALCN (Accession Q6Q760). Extracellular, S1-S2 domain III.
Accession (Uniprot) Number Q6Q760
Gene ID 266760
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_11120881.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, lci, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 4), mouse heart (lanes 2 and 5) and SH-SYS5 cell (lanes 3 and 6) lysates:
    Western blot analysis of rat brain (lanes 1 and 4), mouse heart (lanes 2 and 5) and SH-SYS5 cell (lanes 3 and 6) lysates:
    1-3. Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022), (1:200).
    4-6. Anti-NALCN/VGCNL1 (extracellular) Antibody, preincubated with NALCN/VGCNL1 (extracellular) Blocking Peptide (#BLP-SC022).
Immunohistochemistry
  • Expression of NALCN in rat hypothalamus
    Expression of NALCN in rat hypothalamus
    Immunohistochemical staining of rat hypothalamus using Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022). A. NALCN (green) staining appears in the supra-optic nucleus (SON). B. DAPI is used as the counterstain (blue). White arrows point at the sharp border between the SON and optic tract (OT). C. Merged images of A and B.
Live cell imaging / Immunocytochemistry
  • Expression of NALCN in rat PC12 cells
    Expression of NALCN in rat PC12 cells
    Cell surface detection of NALCN in intact living rat pheochromocytoma PC12 cells. Extracellular staining of cells using Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody. Merged image of extracellular staining with live image of the cells.
References
  1. Lu, B. et al. (2007) Cell 129, 371.
  2. Gilon, P. and Rorsman, P. (2009) EMBO Rep. 10, 963.
  3. Swayne, L.A. et al. (2009) EMBO Rep. 10, 873.
  4. Swayne, L.A. et al. (2010) Islets 2, 54.
Scientific background

The existence of resting K+ conductance has been known for some time. Only recently has the Nalcn (sodium leak channel non-selective protein) been found responsible for the resting Na+ conductance1.

Nalcn is a member of the NaV and CaV channel family which have four homologous repeats of six transmembrane domains. One structural difference is that Nalcn is less positively charged in the voltage sensing segment1. The protein forms voltage-independent non-inactivating channel which is permeable to Na+, K+ and Ca2+ ions1,2.

Evidence suggests that Nalcn is a non-redundant and thus essential channel since knock-out mice display disrupted respiratory rhythm and die within 24 hours of birth. In addition, isolated neurons from these mice have decreased excitability1.

The channel is extensively expressed in the central nervous system1, heart, pituitary and adrenal glands and pancreatic islet cells2,3.

In pancreatic islets, Nalcn is activated by acetylcholine via its physical association with M3 muscarinic receptor, and potentiates insulin secretion induced by glucose uptake2-4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 27/06/2021

Anti-NALCN/VGCNL1 (extracellular) Antibody (#ASC-022) is a highly specific antibody directed against an epitope of rat Sodium leak channel non-selective protein. The antibody can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize NALCN from mouse, rat, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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