Anti-Somatostatin Receptor Type 5 (extracellular) Antibody

SST5, SSTR5
    Cat #: ASR-005
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide CEPLSLASTPSWNAS, corresponding to amino acid residues 2-15 of rat somatostatin receptor type 5 (accession P30938). Extracellular, N-terminus.
    Accession (Uniprot) Number P30938
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 12/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, lci, wb
    May also work in: ifc, ip
    Western blot
    Western blot analysis of rat brain lysates:
    1. Anti-Somatostatin Receptor Type 5 (extracellular) Antibody (#ASR-005), (1:200).
    2. Anti-Somatostatin Receptor Type 5 (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of SSTR5 in mouse cerebellum
    Immunohistochemical staining of Somatostatin receptor 5 (SST5R) in mouse cerebellum using Anti-Somatostatin Receptor Type 5 (extracellular) Antibody (#ASR-005). A. SST5R (red) appears in Purkinje cells (horizontal arrows) and in molecular layer interneurons (vertical arrows). B. Parvalbumin (green) appears in the Purkinje cells and their processes. C. merge of SST5R and parvalbumin demonstrates co-localization in Purkinje cells (horizontal arrows) but not in the molecular layer (vertical cells). DAPI is used as the counterstain (blue).
    Live cell imaging / Immunocytochemistry
    Expression of SSTR5 in human HT-29 cells
    Immunocytochemical staining of intact living human colorectal adenocarcinoma (HT-29) cells. A. Extracellular staining of cells with Anti-Somatostatin Receptor Type 5 (extracellular) Antibody (#ASR-005), (1:50), (red). B. Merged image of A with live view of cell.
    References
    1. Patel, Y.C. (1999) Front. Neuroendocrin20, 157.
    2. Weckbecker, G. et al. (2003) Nat. Rev. Drug Discov2, 999.
    3. Culler, M.D. et al. (2002) Ann. Endocrinol. (Paris) 63, 2S5.
    4. Corleto, V.D. et al. (2004) Dig. Liver Dis36, S8.
    5. Yang, S.K. et al. (2007) Am. J. Physiol292, E1863
    Scientific background

    Somatostatin (SST) is a small cyclic peptide that was first identified as a powerful inhibitor of the secretion of various hormones including growth hormone (GH), thyroid-stimulating hormone (TSH) and prolactin from the pituitary, as well as practically every major hormone from the intestinal tract. SST consists of two major bioactive forms, SST-14 and the N-terminus extended peptide SST-28, that can be produced by a wide variety of neuroendocrine, inflammatory and immune cells. In target cells, SST induces a variety of physiological functions that include neuromodulation, cell secretion, cell proliferation and smooth muscle contractility.

    SST acts on its multiple cell targets via a family of six receptors that originate from five genes: SSTR1, SSTR2a, SSTR2b, SSTRR3SSTR4, SSTR5. The SSTRs are members of the G-protein coupled receptor superfamily and they modulate cell response via multiple second messenger systems such as inhibition of adenylate cyclase, modulation of conductance of ion channels and protein dephosphorylation.

    In contrast to SSTR1-4 that bind SST-14 and SST-28 with similar affinity, SSTR5 has a much greater affinity to SST-28 over SST-14.

    SSTRs are widely expressed in both brain and peripheral tissues but with receptor subtype variations between the different cell types.
    Strong SSTR expression has been detected in a variety of human tumors including pancreatic, breast, lung and prostate cancers. In fact, SST has been shown to inhibit the growth of various normal and tumor cells. On this basis, several long lasting SST analogs have been developed and are being tested for use in several pathologies such as various cancers, acromegaly, immunoproliferative disorders, diabetic retinopathy, epilepsy and pain.

    SSTR5 is expressed in a variety of tissues and cells, including pancreas, colonic mucosa, small intestine, heart, and vascular smooth muscle cells.
    SSTR5 has been shown to mediate growth inhibition and cell proliferation, and to play an important role in cancer growth regulation as one of the most potent inhibitory receptors.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 05/11/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat SSTR5. Anti-Somatostatin Receptor Type 5 (extracellular) Antibody (#ASR-005), can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize SSTR5 in human, mouse and rat samples.

    For research purposes only, not for human use