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Anti-S1PR2 (EDG5) Antibody

Sphingosine 1-phosphate receptor 2, S1P2, Endothelial differentiation G-protein coupled receptor 5, Lysophospholipid receptor B2, LPB2

Cat #: ASR-012
Alternative Name Sphingosine 1-phosphate receptor 2, S1P2, Endothelial differentiation G-protein coupled receptor 5, Lysophospholipid receptor B2, LPB2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide ERQVALAKVKLYGSDKSC, corresponding to amino acid residues 129-146 of mouse S1PR2 (Accession P52592). 2nd intracellular loop.
Accession (Uniprot) Number P52592
Gene ID 14739
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - 17/18 amino acid residues identical; human - 16/18 amino acid residues identical.
RRID AB_10556618.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.95 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat kidney (lanes 1 and 3), rat heart (lanes 2 and 4), human SH-SY5Y brain neuroblastoma (lanes 5 and 7) and Human MS-1  neuroendocrine skin carcinoma cell (lanes 6 and 8) lysates:
    Western blot analysis of rat kidney (lanes 1 and 3), rat heart (lanes 2 and 4), human SH-SY5Y brain neuroblastoma (lanes 5 and 7) and Human MS-1  neuroendocrine skin carcinoma cell (lanes 6 and 8) lysates:
    1,2,5,6. Anti-S1PR2 (EDG5) Antibody (#ASR-012), (1:200).
    3,4,7,8. Anti-S1PR2 (EDG5) Antibody, preincubated with S1PR2/EDG5 Blocking Peptide (#BLP-SR012).
Immunohistochemistry
  • Expression of Sphingosine 1-phosphate receptor 2 in rat lung
    Expression of Sphingosine 1-phosphate receptor 2 in rat lung
    Immunohistochemical staining of paraffin embedded rat lung sections using Anti-S1PR2 (EDG5) Antibody (#ASR-012), (1:100). S1PR2 is expressed in respiratory epithelium (red arrows). Hematoxilin is used as the counterstain.
References
  1. Pyne, S. and Pyne, N.J. (2000) Biochem. J. 349, 385.
  2. Hla, T. et al. (2001) Science 294, 1875.
  3. Takuwa, Y. et al. (2002) J. Biochem. 131, 767.
  4. Rosen, H. et al (2009) Annu. Rev. Biochem. 78, 743.
  5. Rosen, H and Liao, J. (2003) Curr. Opin. Chem. Biol. 7, 461.
Scientific background

Lysophospholipids constitute a group of important lipid mediators; lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (S1P), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2.

Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG); S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3.

Sphingosine 1-phosphate receptor 2 (S1PR2) is ubiquitously expressed. S1PR2 mRNA was detected in the brain, heart, spleen, liver, lung, thymus, kidney and skeletal muscle4. Unlike S1PR1, knockout of SIPR2 gene is not lethal5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 06/11/2022

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of mouse sphingosine 1-phosphate receptor 2. Anti-S1PR2 (EDG5) Antibody (#ASR-012) can be used in western blot and immunohistochemistry applications. It has been designed to recognize S1PR2 from mouse, rat and human samples.

For research purposes only, not for human use
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