- Peptide ERQVALAKVKLYGSDKSC, corresponding to amino acid residues 129-146 of mouse S1PR2 (Accession P52592). 2nd intracellular loop.
- Western blot analysis of rat kidney (lanes 1 and 3), rat heart (lanes 2 and 4), human SH-SY5Y brain neuroblastoma (lanes 5 and 7) and Human MS-1 neuroendocrine skin carcinoma cell (lanes 6 and 8) lysates:1,2,5,6. Anti-S1PR2 (EDG5) Antibody (#ASR-012), (1:200).
3,4,7,8. Anti-S1PR2 (EDG5) Antibody, preincubated with S1PR2/EDG5 Blocking Peptide (#BLP-SR012).
- Expression of Sphingosine 1-phosphate receptor 2 in rat lungImmunohistochemical staining of paraffin embedded rat lung sections using Anti-S1PR2 (EDG5) Antibody (#ASR-012), (1:100). S1PR2 is expressed in respiratory epithelium (red arrows). Hematoxilin is used as the counterstain.
Lysophospholipids constitute a group of important lipid mediators; lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (S1P), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2.
Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG); S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3.
Sphingosine 1-phosphate receptor 2 (S1PR2) is ubiquitously expressed. S1PR2 mRNA was detected in the brain, heart, spleen, liver, lung, thymus, kidney and skeletal muscle4. Unlike S1PR1, knockout of SIPR2 gene is not lethal5.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of mouse sphingosine 1-phosphate receptor 2. Anti-S1PR2 (EDG5) Antibody (#ASR-012) can be used in western blot and immunohistochemistry applications. It has been designed to recognize S1PR2 from mouse, rat and human samples.
- Anti-S1PR1 (EDG1) (extracellular) Antibody (#ASR-011)
- Anti-S1PR1 (EDG1) (extracellular)-FITC Antibody (#ASR-011-F)
- Anti-S1PR3 (EDG3) (extracellular) Antibody (#ASR-013)
- Anti-S1PR3 (EDG3) (extracellular)-ATTO Fluor-488 Antibody (#ASR-013-AG)
- Anti-S1PR3 (EDG3) (extracellular)-FITC Antibody (#ASR-013-F)
- Anti-S1PR5/EDG8 (extracellular) Antibody (#ASR-015)