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Anti-SynDIG1 Antibody

Synapse differentiation-inducing gene protein 1, Transmembrane Protein 90B, TMEM90B, Dispanin subfamily C member 2, DSPC2

Cat #: AGC-049
Alternative Name Synapse differentiation-inducing gene protein 1, Transmembrane Protein 90B, TMEM90B, Dispanin subfamily C member 2, DSPC2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RSPTKDSLEYPDGK, corresponding to amino acid residues 119-132 of rat SynDIG1 (Accession Q58DZ9). Intracellular, N-terminus.
Accession (Uniprot) Number Q58DZ9
Gene ID 362235
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2756603.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1,2. Anti-SynDIG1 Antibody (#AGC-049), (1:200).
    3,4. Anti-SynDIG1 Antibody, preincubated with SynDIG1 Blocking Peptide (#BLP-GC049).
  • Western blot analysis of human SH-SY5Y neuroblastoma cell line:
    Western blot analysis of human SH-SY5Y neuroblastoma cell line:
    1. Anti-SynDIG1 Antibody (#AGC-049), (1:400).
    2. Anti-SynDIG1 Antibody, preincubated with SynDIG1 Blocking Peptide (#BLP-GC049).
References
  1. Chenaux, G. et al. (2016) eNeuro 3, 5.
  2. Kaur, I. et al. (2016) J. Neurosci. 36, 7562.
Scientific background

SynDIG1 (Synapse differentiation induced gene 1) is a brain-specific, highly conserved type II integral membrane protein detected at excitatory synapses. The protein is responsible for the regulation of excitatory synapse number and strength in hippocampal neurons. The protein plays an important role during synapse development in regulating AMPA receptor (AMPAR) and PSD-95 content at excitatory synapses1,2.

SynDIG family includes four members: SynDIG1-4. SynDIG1 structure includes a large intracellular N-terminal region followed by a single transmembrane domain and a second hydrophobic segment that does not span the membrane. The membrane-associated region forms a three-helical bundle with two cysteine residues located at positions 191 and 192 in the juxta-transmembrane region exposed to the cytoplasm1,2.

In studies conducted on mice, deletion of endogenous SynDIG1 in the mouse brain results in a significant decrease in excitatory synapse maturation, but not in synapse composition. Thus, SynDIG1 is not required for synaptogenesis in vivo1. Contrarily, overexpression of SynDIG1 in cultures of dissociated rat hippocampal neurons shows a significant increase in excitatory synapse strength and number.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-SynDIG1 Antibody (#AGC-049) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize SynDIG1 from human, rat, and mouse samples.

For research purposes only, not for human use
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