Anti-SynDIG1 Antibody

Synapse differentiation-inducing gene protein 1, Transmembrane Protein 90B, TMEM90B, Dispanin Subfamily C Member 2, DSPC2
  • New
Cat #: AGC-049
Sizes: 25 µl, 50 µl, 0.2 ml
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)RSPTKDSLEYPDGK, corresponding to amino acid residues 119-132 of rat SynDIG1 (Accession Q58DZ9). Intracellular, N-terminus.
    Accession (Uniprot) Number Q58DZ9
    Gene ID 362235
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.w
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
    Western blot
    Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    1, 2. Anti-SynDIG1 Antibody (#AGC-049), (1:200).
    3, 4. Anti-SynDIG1 Antibody, preincubated with the control peptide antigen.
    Western blot analysis of human SH-SY5Y neuroblastoma cell line:
    1. Anti-SynDIG1 Antibody (#AGC-049), (1:400).
    2. Anti-SynDIG1 Antibody, preincubated with the control peptide antigen.
    References
    1. Chenaux, G. et al. (2016) eNeuro 3, 5.
    2. Kaur, I. et al. (2016) J. Neurosci. 36, 7562.
    Scientific background

    SynDIG1 (Synapse Differentiation Induced Gene 1) is a brain-specific, highly conserved type II integral membrane protein detected excitatory synapses. The protein is responsible for the regulation of excitatory synapse number and strength in hippocampal neurons. The protein plays an important role during synapse development in regulating AMPA receptor (AMPAR) and PSD-95 content at excitatory synapses1,2.

    SynDIG family includes four members: SynDIG1-4. SynDIG1 structure includes a large intracellular N-terminal region followed by a single transmembrane domain and a second hydrophobic segment that does not span the membrane. The membrane-associated region forms a three-helical bundle with two cysteine residues located at positions 191 and 192 in the juxta-transmembrane region exposed to the cytoplasm1,2.

    In studies conducted on mice, deletion of endogenous SynDIG1 in the mouse brain results in a significant decrease in excitatory synapse maturation, but not in synapse composition. Thus, SynDIG1 is not required for synaptogenesis in vivo1. Contrarily, overexpression of SynDIG1 in cultures of dissociated rat hippocampal neurons shows a significant increase in excitatory synapse strength and number.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 12/04/2018

    Anti-SynDIG1 Antibody (#AGC-049) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize SynDIG1 from human, rat, and mouse samples.

    For research purposes only, not for human use