- Peptide RDRLPDLTACRKSDDGDN, corresponding to amino acid residues 2-19 of rat Syntaxin 2 (Accession P50279). N-terminus.
- Western blot analysis of rat brain membranes:1. Anti-Syntaxin 2 Antibody (#ANR-008), (1:600).
2. Anti-Syntaxin 2 Antibody, preincubated with Syntaxin 2 Blocking Peptide (#BLP-NR008).
- Rat brain sections.
- Jahn, R. and Scheller, R.H. (2006) Nat. Rev. Mol. Cell Biol. 7, 631.
- Südhof, T.C. and Rothman, J.E. (2009) Science 323, 474.
- Chen, D. et al (2000) Blood 95, 921.
- Hutt, D.M. et al (2005) J. Biol. Chem. 280, 20197.
- Hansen, N.J. et al (1999) J. Biol. Chem. 274, 22871.
- Radisky, D.C. et al (2003) Trends Cell. Biol. 13, 426.
Syntaxin 2 is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60–70 amino acids that are arranged in heptad repeats1,2.
SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2.
SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion.
Syntaxin 2, like most SNAREs, is a type IV membrane protein with a relatively large N-terminus containing the SNARE motif located in the cytoplasmic side and a transmembrane domain located close to the C-terminus that functions as an anchor1,2.
Syntaxin 2 functions as a plasma membrane protein t-SNARE, which together with vesicular v-SNARE proteins is involved in regulated exocytosis in cells such as platelets, sperm cells and pancreatic acinar cells3-5. Intriguingly, Syntaxin 2 has been also identified as epimorphin, an extracellular membrane protein implicated in branching morphogenesis, a developmental process essential for the formation of many organs, including mammary gland, lung, kidney, and salivary gland6.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Syntaxin 2. Anti-Syntaxin 2 Antibody (#ANR-008) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Syntaxin 2 from rat, mouse, and human samples.
- Rat pancreas lysate (1:600).
Satoh, K. et al. (2016) Am. J. Physiol. 310, G399.