Anti-Taurine Transporter (SLC6A6) Antibody

Sodium- and chloride-dependent taurine transporter, TauT
    Cat #: AGT-006
    Alternative Name Sodium- and chloride-dependent taurine transporter, TauT
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide (C)TPREPNRWAVEREG, corresponding to amino acid residues 581-594 of rat TauT (Accession P31643). Intracellular, C-terminus.
    • Anti-Taurine Transporter (SLC6A6) Antibody
    Accession (Uniprot) Number P31643
    Gene ID 29464
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human – identical.
    RRID AB_2756638.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
    Western blot
    • Anti-Taurine Transporter (SLC6A6) Antibody
      Western blot analysis of mouse brain membranes:
      1. Anti-Taurine Transporter (SLC6A6) Antibody (#AGT-006), (1:200).
      2. Anti-Taurine Transporter (SLC6A6) Antibody, preincubated with the negative control antigen.
    • Anti-Taurine Transporter (SLC6A6) Antibody
      Western blot analysis of rat small intestine lysate:
      1. Anti-Taurine Transporter (SLC6A6) Antibody (#AGT-006), (1:200).
      2. Anti-Taurine Transporter (SLC6A6) Antibody, preincubated with the negative control antigen.
    • Anti-Taurine Transporter (SLC6A6) Antibody
      Western blot analysis of human HL-60 promyelocytic leukemia cell lysate (lanes 1 and 3) and human HT-29 colon adenocarcinoma cell lysate (lanes 2 and 4):
      1,2. Anti-Taurine Transporter (SLC6A6) Antibody (#AGT-006), (1:200).
      3,4. Anti-Taurine Transporter (SLC6A6) Antibody, preincubated with the negative control antigen.
    1. Ripps, H. and Shen, W. (2012) Mol. Vis. 18, 2673.
    2. Bondareva, O.M. et al. (2008) Pharm. Chem. J. 42, 142.
    3. Zelikovic, I. and Chesney, R.H. (1989) Kidney Int. 36, 351.
    4. Han, X. et al. (1996) J. Am. Soc. Nephrol. 7, 2088.
    5. Schaffer, S.W. et al. (2010) J. Biomed. Sci. 17, S2.
    Scientific background

    Taurine (2-aminoethane-sulfonice acid) is one of the most abundant amino acids found in the human body. It is involved in cell regulation and modulates the concentration of intracellular free calcium. It also plays a substantial role in many other functions varying from neurotransmission in the central nervous system to promotion of cell differentiation in the brain to cytoprotection against oxidative stress1.

    Taurine is not used for protein synthesis, and in addition to its uptake in nutrition, it can be synthesized in pancreatic mammalian cells by cysteine dioxygenase and sulfinoalanine decarboxylase enzymes2.

    Taurine is found mainly in organs such as the brain, retina and muscle tissue.

    The uptake of taurine is conducted through the taurine transporter (TauT). The energy for passage through the transporter is derived by Na+ and Cl- concentration similar to the uptake of other amino acids3. TauT also transports GABA, albeit with a lower affinity. The transporter has twelve transmembrane domains and intracellular N- and C-termini.

    TauT expression is regulated by cAMP which was found to increase its regulation in MDCK cells and also by PKC via means of post-translational modification. PKC activation leads to reduced taurine transport4. PKC is believed to phosphorylate the highly conserved serine-322 position in the transporter.

    Taurine deficiency is linked to cardiomyopathies in the hearts of cats and to a decrease in cardiac muscle sensitivity to calciumand better understanding of its transport may improve research of these diseases.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat TauT. Anti-Taurine Transporter (SLC6A6) Antibody (#AGT-006) can be used in western blot analysis. It has been designed to recognize TauT from mouse, rat and human samples.

    For research purposes only, not for human use
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