Anti-Thromboxane A2 Receptor Antibody

Prostanoid TP Receptor, TXA2-R, TBXA2R
  • New
Cat #: APR-069
  • Lyophilized Powder
  • Antigen Incl.
  • QC Tested
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)HAREATQRPRD(S)EVE, corresponding to amino acid residues 225 - 239 of rat Thromboxane A2 Receptor (Accession P34978). 3rd intracellular loop.
    Accession (Uniprot) Number P34978
    Gene ID 24816
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat – 14/15 amino acid residues identical; mouse – 13/15 amino acid residues identical; human – 12/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
    Western blot
    Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):
    1, 2. Anti-Thromboxane A2 Receptor Antibody (#APR-069), (1:200).
    3, 4. Anti-Thromboxane A2 Receptor Antibody, preincubated with the control peptide antigen.
    Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 2 and 4):
    1, 2. Anti-Thromboxane A2 Receptor Antibody (#APR-069), (1:200).
    3, 4. Anti-Thromboxane A2 Receptor Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of Thromboxane A2 Receptor in mouse cortex and hippocampus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Thromboxane A2 Receptor Antibody (#APR-069), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Thromboxane A2 Receptor immunoreactivity (green) is observed in mouse parietal cortex neurons (arrows). B. Thromboxane A2 Receptor immunoreactivity (green) is detected in neurons (horizontal arrows) of mouse hippocampal CA3 pyramidal layer (P). Cell nuclei are stained with DAPI (blue).
    References
    1. Yan, A. et al. (2017) Mol. Med. Rep. 16, 2491.
    2. Zhao, Z. et al. (2017) Oncotarget 8, 30030.
    3. Kinsella, B.T. (2016) Wilms Tumor, Brisbane (AU): Codon Publications, Chapter 11.
    Scientific background

    Thromboxane A2 (TxA2) is a prostanoid that is synthesized from arachidonic acid and plays an important role in haemostasis, a process regulating platelet aggregation and vascular tone. Thromboxane A2 binds to the thromboxane A2 receptor (TXA2R) to induce strong vasoconstriction in renal arteries.

    Thromboxane A2 receptor is a seven-transmembrane G-protein coupled receptor localized on the cell membrane and in intracellular compartments. Thromboxane A2 receptor is expressed in the central nervous system in several types of cells, such as microglia, oligodendrocytes and astrocytes. Imbalances in the levels of the receptor is widely implicated in several cardiovascular, pulmonary, renal and prostate pathologies1-3.

    Thromboxane A2 receptor mediates vasoconstriction and ischemia and its activation is implicated in atherosclerosis and inflammation. Activation of the receptor induces endothelial cell apoptosis by inhibiting Akt phosphorylation and also inhibits endothelial cell migration and angiogenesis by decreasing Akt and eNOS phosphorylation1-3.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 20/08/2018

    Anti-Thromboxane A2 Receptor Antibody (#APR-069) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize TXA2-R from rat, mouse, and human samples.

    For research purposes only, not for human use