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Anti-TrkC (extracellular) Antibody

Neurotrophic tyrosine kinase receptor 3, NT-3 growth factor receptor, GP145-TrkC, NTRK3
Cat #: ANT-020
Alternative Name Neurotrophic tyrosine kinase receptor 3, NT-3 growth factor receptor, GP145-TrkC, NTRK3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)GHFLKEPFPESTD, corresponding to amino acid residues 393-405 of rat TrkC (Accession Q03351). Extracellular, N-terminus.
Accession (Uniprot) Number Q03351
Gene ID 29613
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_2341011.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, lci, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of HEK-TrkC transfected cells (lanes 1 and 3) and HEK untransfected cells (lanes 2 and 4) lysates:
    Western blot analysis of HEK-TrkC transfected cells (lanes 1 and 3) and HEK untransfected cells (lanes 2 and 4) lysates:
    1,2. Anti-TrkC (extracellular) Antibody (#ANT-020), (1:400).
    3,4. Anti-TrkC (extracellular) Antibody, preincubated with TrkC (extracellular) Blocking Peptide (#BLP-NT020).
Immunohistochemistry
  • Expression of TrkC in rat dorsal root ganglia
    Expression of TrkC in rat dorsal root ganglia
    Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-TrkC (extracellular) Antibody (#ANT-020), (1:100). A. TrkC labeling (red) appears in cell bodies. Note that the nerve fibers are not stained. B. Nuclear staining (blue) was visualized using Hoechst 33342. C. Merge of A and B.
Live cell imaging / Immunocytochemistry
  • Expression of TrkC in rat PC12 cells
    Expression of TrkC in rat PC12 cells
    Cell surface detection of TrkC in live intact rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-TrkC (extracellular) Antibody (#ANT-020), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Nuclear staining using the cell permeable dye Hoechst 33342 (blue). C. Merge image of A and B.
References
  1. Webster, N.J.G. and Pirrung, M.C. (2008) BMC Neurosci. 9, S1.
  2. Quartu, M. et al. (2003) Int. J. Dev. Neurosci. 21, 309.
  3. Aoki, C. et al. (2000) J. Neurosci. Res. 59, 454.
  4. Yan, Q. et al. (1997) J. Comp. Neurol. 378, 135.
  5. Patapoutian, A. and Reichardt, L.F. (2001) Curr. Opin. Neurobiol. 11, 272.
  6. Schecterson, L.C. and Bothwell, M. (2010) Develop. Neurobiol. 70, 332.
  7. Grimes, M.L. et al. (1996) J. Neurosci. 16, 7950.
Scientific background

BDNF and NT-4 belong to the neurotrophin family which also includes NGF and NT-3. These neurotrophins bind two groups of receptors. The p75NTR receptor is common to all four neurotrophins and is a member of the tumor necrosis factor receptor family. The tropomyosin-related kinase (Trk) receptors are receptor tyrosine kinases (RTKs) and three receptors form this family: TrkA, TrkB, and TrkC1.

As mentioned above, the p75NTR receptor binds to all neurotrophins with similar affinities while the Trk receptors are the ones to display the selectivity for the neurotrophins. TrkA is activated by NGF binding, TrkB by that of BDNF and NT-4, while TrkC is stimulated by the binding of NT-3 1.

All three Trk receptors are highly expressed in the mammalian brain in very distinct regions and are also expressed in the peripheral nervous system2-4. Cholinergic neurons in the basal forebrain exclusively express TrkA, while TrkB and TrkC are highly expressed in the hippocampus. Motor and sensory neurons in the peripheral nervous system express Trk receptors. Interestingly, Trk receptors are not essential for development, but knockout mice die shortly after birth. Indeed, TrkB-deficient mice demonstrate a significant decrease in motor neurons and synaptogenesis1.

Trk receptors have many motifs in the extracellular region, including cell-adhesion domains, three tandem leucine rich motifs flanked by two clusters of cysteines. In the membrane proximal region of the receptor there are also two immunoglobulin-like domains5. The binding of neurotrophins to Trk receptors promotes receptor dimerization resulting in kinase activation. Activated Trk receptors then phosphorylate a cascade of signaling molecules including the Ras/ERK, PI3K/Akt pathways and PLC-γ1. Activated Trk receptors also create internal docking sites for other signaling adaptor proteins to bind to5. Splice variants of TrkA, TrkB and TrkC have been observed. These splice isoforms are mainly affected in the tyrosine kinase domain of the receptor lying in the cytoplasm. Endocytosis is an important signaling trait of Trk receptors.

Following neurotrophin binding to the Trk receptor, the receptor complex is then internalized via endocytosis in order to terminate signaling. However, in the axonal compartment of neurons the internalization process of the neurotrophin complexed to the receptor is part of the signaling process and is important for activating transcription processes in the nucleus6,7.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 16/08/2020

Anti-TrkC (extracellular) Antibody (#ANT-020) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize TrkC from rat, mouse, and human samples.

For research purposes only, not for human use
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