Anti-TRPM3 (extracellular) Antibody

Transient receptor potential cation channel subfamily M member 3, Long transient receptor potential channel 3, LTrpC3, Melastatin-2, MLSN2
    Cat #: ACC-050
    Alternative Name Transient receptor potential cation channel subfamily M member 3, Long transient receptor potential channel 3, LTrpC3, Melastatin-2, MLSN2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide KNKDDMPYMSQAQEIH(C), corresponding to amino acid residues 816-831 of human TRPM3 (Accession Q9HCF6). 1st extracellular loop.
        Anti-TRPM3 (extracellular) Antibody
    Accession (Uniprot) Number Q9HCF6
    Gene ID 80036
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - 15/16 amino acid residues identical.
    RRID AB_10918820.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*, lci*
      • Anti-TRPM3 (extracellular) Antibody
        Western blot analysis of rat brain membrane (lanes 1 and 4), rat brain lysate (lanes 2 and 5) and mouse brain lysate (lanes 3 and 6):
        1-3. Anti-TRPM3 (extracellular) Antibody (#ACC-050), (1:200).
        4-6. Anti-TRPM3 (extracellular) Antibody, preincubated with the negative control antigen.
      • Mouse optic nerves sections (Papanikolaou, M. et al. (2017) Brain Struct. Funct. 222, 2993.).
      • Mouse astrocyte culture (Papanikolaou, M. et al. (2017) Brain Struct. Funct. 222, 2993.).
    References
      • Transient receptor potential (TRP) channels are relatively non-selective ion channels enabling the exchange of cations down their electrochemical gradient. This exchange enables the intracellular rise in Na+ and Ca2+ concentration and ultimately in the cell membrane depolarization, important for action potential propagation and muscle contraction1. They are activated by an extremely broad range of stimuli namely, temperature, voltage, pH, endocrine factors as well as signaling molecules2.

        The TRP channel family is composed of 28 members divided in 7 subgroups: TRPV, TRPC, TRPM, TRPA, TRPN, TRPP and TRPML. All members of the TRP family have 6 transmembrane (TM) domains, and a pore domain between the fifth (S5) and sixth (S6) transmembrane domains. In general, TRP channels enable the passage of either Na+ or Ca2+ ions with little or no preference. However, some channels do exhibit some selectivity. Also, TRP channels do not display the positive charges in the S4 voltage-sensing domain like most voltage sensitive channels, although they do display voltage dependency3. In addition, TRP channels have in the C-terminal intracellular region to the S6 domain a TRP domain comprising 25 amino acids that is more or less conserved among most TRP channels. Within the TRP domain, there is a TRP box composed of six amino acids, and TRP box 2 – a proline rich domain1,3. The TRP domain seems to be responsible for the binding of PIP2, a phospholipid important for the regulation of channel activity4.

        The TRPM subfamily consists of 8 members divided in to three major groups: TRPM1/3, TRPM4/5, and TRPM6/7. TRPM2 and TRPM8 are not included in any of the groups since they are quite different even with respect to each other. TRPM3 is alternatively spliced into active variants5,6. TRPM3a1 and TRPM3a2 in mouse are monovalent and divalent-selective channels, respectively. This differential selectivity strongly suggests that their cellular role depends on the relative expression of each variant type. In addition, both splice variants display constitutive outwardly rectifying currents. They somewhat resemble TRPM6 and TRPM7 channels such that their currents are inhibited by intracellular Mg2+ 1.

        TRPM3 is highly expressed in the kidney, and to a lesser extent in the brain, testis, and spinal cord7.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-TRPM3 (extracellular) Antibody
    Expression of TRPM3 in mouse optic nerves.Bi-Biv. Immunohistochemical staining of mouse optic nerve sections using Anti-TRPM3 (extracellular) Antibody (#ACC-050). TRPM3 staining (green) is highly expressed in astrocytes and co-localizes with GFAP, an astrocyte marker. Bv. Immunocytochemical staining of explant cultures also shows expression of TRPM3 (green).Adapted from Papanikolaou, M. et al. (2017) Brain Struct. Funct. 222, 2993. with permission of Springer Nature publishing.
    Last update: 10/11/2019

    Anti-TRPM3 (extracellular) Antibody (#ACC-050) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize TRPM3 from human, rat, and mouse samples.

    For research purposes only, not for human use
    Citations
      • Mouse optic nerve sections.
        Papanikolaou, M. et al. (2017) Brain Struct. Funct. 222, 2993.
      • Mouse trigeminal ganglion sections.
        Suzuki, A. et al. (2016) Mol. Pain 12, 1.
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