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Anti-TRPM5 Antibody

LTrpC5, MTR1, Transient receptor potential cation channel subfamily M member 5

Cat #: ACC-045
Alternative Name LTrpC5, MTR1, Transient receptor potential cation channel subfamily M member 5
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide GSGKKRGKFVKVPS(C), corresponding to amino acid residues 32-45 of mouse TRPM5 (Accession Q9JJH7). Intracellular, N-terminus.
Accession (Uniprot) Number Q9JJH7
Gene ID 56843
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human - 13/14 amino acid residues identical.
RRID AB_2040252.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat lung membrane (lanes 1 and 2), rat testes (lanes 3 and 4), mouse ms1 pancreas cells (lanes 5 and 7) and human LNCaP prostate cell (lanes 6 and 8) lysates:
    Western blot analysis of rat lung membrane (lanes 1 and 2), rat testes (lanes 3 and 4), mouse ms1 pancreas cells (lanes 5 and 7) and human LNCaP prostate cell (lanes 6 and 8) lysates:
    1,3,5,6. Anti-TRPM5 Antibody (#ACC-045), (1:200).
    2,4,7,8. Anti-TRPM5 Antibody, preincubated with TRPM5 Blocking Peptide (#BLP-CC045).
Immunohistochemistry
  • Expression of TRPM5 in rat tongue
    Expression of TRPM5 in rat tongue
    Immunohistochemical staining of rat tongue paraffin embedded sections using Anti-TRPM5 Antibody (#ACC-045), (1:100). TRPM5 is expressed in discrete groups of cells deep in the sulcus of the papillae. Hematoxilin is used as the counterstain.
  • Expression of TRPM5 in rat testes
    Expression of TRPM5 in rat testes
    Immunohistochemical staining of rat epididymis paraffin embedded sections using Anti-TRPM5 Antibody (#ACC-045), (1:100). TRPM5 is expressed in principal cells within the epididymal epithelium (arrows). Hematoxilin is used as the counterstain.
References
  1. Fleig, A. et al. (2004) Trends. Pharmacol. Sci. 25, 633.
  2. Vazquez, E. et al. (2006) Semin. Cell Dev. Biol. 17, 607.
  3. Kraft, R. et al. (2005) Pflugers. Arch. 451, 204.
  4. Fang, D. et al. (2000) Biochem. Biophys. Res. Commun. 279, 53.
  5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690.
  6. Montell, C. (2005) Sci. STKE. 2005, re3.
Scientific background

The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3

The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM71,2.

The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family1-3

TRPM4 and TRPM5 are the only TRP channels that are permeable to monovalent cations but not to Ca2+. TRPM4 is expressed as at least two alternative spliced isoforms, TRPM4a and TRPM4b which consist of shorter and longer N-terminal regions, respectively. TRPM4a displays little activity, TRPM4b and TRPM5 appear to be directly activated by an increase in intracellular Ca2+ concentration4,5.

TRPM5 is primarily expressed in taste buds and also in stomach, small intestine, uterus and testes2. Regulation of TRPM5 by G-protein-coupled receptors and PLC-mediated IP3 production has been substantiated by the colocalization of TRPM5, of a taste-cell-specific PLC isoform and of receptors for sweet, bitter and amino acid (umami) tastes in taste tissue. It has furthermore been shown that desensitization of TRPM5 following receptor stimulation can be reversed by phosphatidylinositol (4,5)-bisphosphate (PIP2), the substrate of PLC2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Image & Title: Anti-TRPM5 Antibody
Expression of TRPM5 in mouse trachea.Immunohistochemical staining of mouse trachea coronal sections using Anti-TRPM5 Antibody (#ACC-045). TRPM5 staining (purple) is detected in tracheal brush cells and colocalizes with ChAT staining (green).Adapted from Yamashita, J. et al. (2017) PLoS ONE 12, e0189340. with permission of PLoS.
Last update: 06/09/2021

Anti-TRPM5 Antibody (#ACC-045) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TRPM5 from mouse, rat and human samples.

For research purposes only, not for human use

Applications

Citations

Citations
Western blot citations
  1. Mouse transfected melanoma cells. 
    Maeda, T. et al. (2017) Oncotarget 8, 78312.
Immunohistochemistry citations
  1. Human skin melanoma sections.
    Maeda, T. et al. (2017) Oncotarget 8, 78312.
  2. Mouse oral epithelial sections (1:3000).
    Ohmoto, M. et al. (2017) Chem. Senses 42, 547.
  3. Mouse trachea sections.
    Yamashita, J. et al. (2017) PLoS ONE 12, e0189340.
  4. Mouse tongue sections.
    Nishiguchi, Y. et al. (2016) Dev. Biol. 416, 98.
  5. Mouse olfactory tissue sections.
    Goss, G.M. et al. (2016) Develop. Neurobiol. 76, 241.

Specifications

Scientific Background

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