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Anti-TRPM6 (extracellular) Antibody

Transient receptor potential cation channel subfamily M member 6, Channel kinase 2, CHAK2, Melastatin-related TRP cation channel 6

Cat #: ACC-046
Alternative Name Transient receptor potential cation channel subfamily M member 6, Channel kinase 2, CHAK2, Melastatin-related TRP cation channel 6
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide CVKDYDLERGPDEK, corresponding to amino acid residues 802-815 of mouse TRPM6 (Accession Q8CIR4). 1st extracellular loop.
Accession (Uniprot) Number Q8CIR4
Gene ID 225997
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - 13/14 amino acid residues identical; human - 11/14 amino acid residues identical.
RRID AB_10658485.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat kidney (lanes 1 and 4), rat testis (lanes 2 and 5) and mouse kidney (lanes 3 and 6) lysates:
    Western blot analysis of rat kidney (lanes 1 and 4), rat testis (lanes 2 and 5) and mouse kidney (lanes 3 and 6) lysates:
    1-3. Anti-TRPM6 (extracellular) Antibody (#ACC-046), (1:200).
    4-6. Anti-TRPM6 (extracellular) Antibody, preincubated with TRPM6 (extracellular) Blocking Peptide (#BLP-CC046).
Immunohistochemistry
  • Mouse placenta sections (1:50) (Suzuki, Y. et al. (2016) J. Physiol. Sci. 67, 151.).
  • Expression of TRPM6 in rat hippocampus. 
    Expression of TRPM6 in rat hippocampus. 
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-TRPM6 (extracellular) Antibody(#ACC-046)(1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the rat hippocampal CA1 region showed TRPM6 immunoreactivity (green) in in the pyramidal cell layer (P) and apical dendrites (arrows). B. Pre-incubation of the antibody with TRPM6 (extracellular) Blocking Peptide (BLP-CC046), suppressed staining. Cell nuclei are stained with DAPI (blue). 
Indirect flow cytometry
  • Cell surface detection of TRPM6 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: 
    Cell surface detection of TRPM6 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: 
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-TRPM6 (extracellular) Antibody(#ACC-046), (2.5μg) + goat-anti-rabbit-FITC .
References
  1. Ramsey, S.I. et al. (2006) Annu. Rev. Physiol. 68, 619.
  2. Pedersen, S.F. et al. (2005) Cell. Calcium. 38, 233.
  3. Montell, C. (2005) Sci. STKE 2005, re3.
  4. Hilgemann, D.W. et al. (2001) Sci. STKE 2001, re19.
  5. Schmitz, C. et al. (2005) J. Biol. Chem. 280, 37763.
Scientific background

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have putative six-transmembrane domains (TM) with a pore domain between the fifth and the six TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3

According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC (canonical), TRPV (vanilloid) and TRPM (melastatin). To date, three extra subfamilies are also considered to belong to the TRP family; the TRPA, TRPML, and the TRPP.1-4

The TRPM subfamily consists of eight members, TRPM1 to TRPM8, which also can be further subdivided into four subgroups based on their sequence homology: (1)TRPM1 and TRPM3 (2) TRPM6 and TRPM7 (3) TRPM4 and TRPM5 (4) TRPM2 and TRPM8.5

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 10/11/2021

Anti-TRPM6 (extracellular) Antibody (#ACC-046) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TRPM6 from mouse, rat, and human samples.

For research purposes only, not for human use

Applications

Citations

Citations
Western blot citations
  1. HEK 293 transfected cells (1:300).
    Suzuki, Y. et al. (2016) J. Physiol. Sci. 67, 151.
Immunohistochemistry citations
  1. Mouse placenta sections (1:50).
    Suzuki, Y. et al. (2016) J. Physiol. Sci. 67, 151.

Specifications

Scientific Background

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