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- Peptide (C)HQPSLDQPAIPSSKAT, corresponding to amino acid residues 413-428 of rat TRPV2 (Accession Q9WUD2). 1st extracellular loop.
- Western blot analysis of ND7/23 cell line membrane (lanes 1 and 3), RBL lysates (lanes 2 and 4) and rat brain membrane (lanes 5 6):1,2,5. Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039), (1:200).
3,4,6. Anti-TRPV2 (VRL1) (extracellular) Antibody, preincubated with the negative control antigen.
- Immunoprecipitation of rat basophilic leukemia (RBL) cell lysate:1. RBL lysate.
2. Lysate immunoprecipitated with Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039), (6 µg).
3. Lysate immunoprecipitated with pre-immune rabbit serum. The upper arrow indicates TRPV2 while the lower arrow indicates the IgG heavy chain. Western blot analysis was performed with Anti-TRPV2 (VRL1) (extracellular) Antibody.
- Expression of TRPV2 in mouse DRGImmunohistochemical staining of TRPV2 in mouse dorsal root ganglion (DRG) using Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039). A. TRPV2 (green) appears in patches along the perimeter of the DRG (arrows). B. Neurons containing neurofilament 200 (red) are scattered in the DRG, also in patches (arrows). C. A merge of the two panels shows that the spatial distribution of neurofilament 200 and TRPV2 expression overlaps. However, DRGs showing robust expression of neurofilament 200 do not contain TRPV2.
- Cell surface detection of TRPV2 in intact living RBL cells:___ Unstained cells.
___ Cells + Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039).
- Expression of TRPV2 in RBL cellsCell surface detection of TRPV2 in intact living rat basophilic leukemia (RBL) cells with Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039), (1:100), followed by goat anti-rabbit-AlexaFluor-550 secondary antibody (red), (x100).
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TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have six putative transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-termini of all TRPs are intracellular3.
According to IUPHAR, the TRP family is comprised of numerous subfamilies on the basis of sequence homology; TRPC, TRPM, TRPV, TRPA, TRPML, and TRPP1-4. The TRPV subfamily consists of six members, TRPV1-65.
Four members of the TRPV family have been described as thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (<17°C) to noxious heat (>52°C) 6,7. Although it shares around 50% homology with TRPV1, TRPV2 is not activated by capsaicin nor by protons. It has a high temperature threshold of ~52°C and is considered to play an essential role in the perception of high-intensity noxious heat stimulation8-10. TRPV2 is also considered to be a stretch-activated channel and to play a role in skeletal and cardiac muscle degeneration and pain pathway8. The TRPV2 channel is expressed in DRG neurons, different brain regions and non-neuronal tissues such as the spleen, lung and intestine and in components of the immune system5,11,12.
Species reactivity key:
Anti-TRPV2 (VRL1) (extracellular) Antibody (#ACC-039) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, live cell imaging, and indirect flow cytometry applications. It has been designed to recognize TRPV2 from mouse and rat samples.
- Rat brain sections.
Zhang, H. et al. (2016) Eur. J. Neurosci. 44, 2493.
- Anti-TRPV2 (VRL1) Antibody (#ACC-032). This antibody recognizes a different epitope and thus can also be used as a control for detecting TRPV2.
- Anti-Human TRPV2 (VRL1) (extracellular) Antibody (#ACC-139). This antibody recognizes a different epitope and thus can also be used as a control for detecting TRPV2 from human samples only.
- Guinea pig Anti-TRPV2 (VRL1) (extracellular) Antibody (#AGP-033) is raised in guinea pig and can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit. This antibody has been raised against the same epitope as #ACC-039.
- Anti-TRPV2 (VRL1) (extracellular)-FITC Antibody (#ACC-039-F). This FITC-conjugated antibody can be used to detect TRPV2 in live cell flow cytometry.
- Anti-TRPV2 (VRL1) (extracellular)-FITC Antibody (#ACC-039-F). A fluorescent labeled primary antibody. It can be used to detect TRPV2 in live cells.