Anti-TRPV3 (extracellular)-Biotin Antibody

VRL-3, Trpv3
    Cat #: ACC-033-B
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (C)REEEAIPHPLALTHK, corresponding to amino acid residues 464-478 of human TRPV3 (Accession Q8NET8). 1st extracellular loop.
    Accession (Uniprot) Number Q8NET8
    Gene ID 162514
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - 13/15 amino acid residues identical; mouse - 12/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label Biotin.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, lci, wb
    May also work in: fc, ih, ip
    Western blot
    Western blot analysis of rat brain lysate:
    1. Anti-TRPV3 (extracellular)-Biotin Antibody (#ACC-033-B), (1:5000)
    2. Anti-TRPV3 (extracellular)-Biotin Antibody preincubated with the control antigen.
    Live cell imaging / Immunocytochemistry
    Expression of TRPV3 in rat PC12 cells
    Immunocytochemical staining of intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-TRPV3 (extracellular)-Biotin Antibody (#ACC-033-B), (1:50), (red). B. Merge of A with the live view of the cell.
    References
    1. Montell, Cet al. (2002) Mol. Cell. 9, 229.
    2. Clapham, D.E. (2003) Nature 426, 517.
    3. Moran, M.Met al. (2004) Curr. Opin. Neurobiol14, 362.
    4. Clapham, D.Eet al. (2003) Pharmacol. Rev55, 591.
    5. Gunthorpe, M.Jet al. (2002) Trends. Pharmacol. Sci23, 183.
    6. Tominaga, M. and Caterina, M.J. (2004) J. Neurobiol61, 3.
    7. Voets, Tet al. (2004) Nature 430, 748.
    8. Xiao, R. et al. (2008) J. Biol. Chem. 283, 6162.
    Scientific background

    TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have six putative transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3

    According to IUPHAR, the TRP family is composed of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three additional subfamilies are also considered to belong to the TRP family: the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5

    Four members of the TRPV family have been described as a thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (<17°C) to noxious heat (>52°C).6,7

    TRPV3 is highly expressed in skin keratinocytes. It forms a nonselective cation channel that is activated by hot temperatures in the innocuous and noxious range. The channel is also expressed in oral and nasal cavities where it has been postulated to play a role in flavor sensation. TRPV3 is also implicated as a molecular target of some allergens and skin sensitizers.8

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 22/10/2018

    Anti-TRPV3 (extracellular) Antibody (#ACC-033) is a highly specific antibody directed against an extracellular epitope of human TRPV3. The antibody can be used for western blot analysis, immunohistochemical and immunocytochemical applications. As the antibody recognizes an extracellular epitope, it can be used as a tool to detect TRPV3 in live cells. It has been designed to recognize TRPV3 from human, rat and mouse samples.

    Anti-TRPV3 (extracellular)-Biotin Antibody (#ACC-033-B) is directly labeled with biotin. Strepavidin tagged with HRP or with a fluorescent probe can then be used to detect the protein. The biotin/strepavidin system is ideal for minimizing cross-reactivity when same species antibodies are simultaneously used. Anti-TRPV3 (extracellular)-Biotin Antibody has been tested in western blot and live cell imaging and is specially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use
    Citations
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