- Peptide (C)RAKPRPAGSLKDLE, corresponding to amino acid residues 399-412 of rat AVPR1B (Accession P48974). Intracellular, C-terminus.
- Western blot analysis of rat lung (lanes 1 and 3) and mouse kidney (lanes 2 and 4) lysates:1,2. Anti-Vasopressin V1B Receptor (AVPR1B) Antibody (#AVR-011), (1:200).
3,4. Anti-Vasopressin V1B Receptor (AVPR1B) Antibody, preincubated with Vasopressin V1B Receptor/AVPR1B Blocking Peptide (#BLP-VR011).
Vasopressin V1b receptor (also called V3R) is a G protein-coupled receptor (GPCR) of the Gq type, thus activating the phospholipase C signaling pathway upon activation. It is part of a family of GPCRs triggered by vasopressin that further include V1R, V2R, oxytocin receptors and purinergic receptors. While V1R and V3R are Gq GPCRs, V2R is coupled to the Gs protein and activates adenylate cyclase1,2.
The V1b receptor has an intracellular C-terminal, seven transmembrane domains, and an extracellular N-tail; the transmembrane and extracellular domains are responsible for interacting with agonists and to signal for activation of the coupled Gq receptor1,2.
The V1b receptor is mainly detected in the corticotrophs of the anterior pituitary gland in the brain where it regulates the release of adrenocorticotrophic hormone (ACTH) upon stimulation of vasopressin-corticotrophin-releasing hormone conjugates2.
V1b KO mice show that the receptor is critical for the functionality of the hypothalamic-pituitary-adrenal axis, and demonstrate its importance in regulating glucose levels. Recent studies have also suggested that lactating rats treated with V1b antagonists, were less nurturing and similarly aggressive compared with untreated rats and that mice with reduced expression of V1b and V1a receptors have shown resistance to jet-lag1,3,4.
Species reactivity key:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Arginine vasopressin receptor 1B. Anti-Vasopressin V1B Receptor (AVPR1B) Antibody (#AVR-011) can be used in western blot analysis. It has been designed to recognize V1bR from rat and mouse samples. The antibody is not recommended for human samples.