Anti-VGLUT3 Antibody

Vesicular glutamate transporter 3, SLC17A8
    Cat #: AGC-037
    Alternative Name Vesicular glutamate transporter 3, SLC17A8
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
    Immunogen
    • Peptide (C)ELNHEAFVSPRKK, corresponding to amino acid residues 533-545 of rat VGLUT3 (Accession Q7TSF2). Cytoplasmic, C-terminus.
    • Anti-VGLUT3 Antibody
    Accession (Uniprot) Number Q7TSF2
    Gene ID 266767
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 12/13 amino acid residues identical; human - 10/13 amino acid residues identical.
    RRID AB_2340952.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
    Western blot
    • Anti-VGLUT3 Antibody
      Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes:
      1,2. Anti-VGLUT3 Antibody (#AGC-037), (1:500).
      3,4. Anti-VGLUT3 Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-VGLUT3 Antibody
      Expression of VGLUT3 in rat and mouse brain
      Immunohistochemical staining of perfusion-fixed frozen rat and mouse brain sections using Anti-VGLUT3 Antibody (#AGC-037), (1:400), followed by anti-rabbit-Cy3 antibody (red). Nuclei were labeled with DAPI and appear in blue. A. VGLUT3 staining in rat piriform cortex appears as grains along layer 2 (arrows). B. VGLUT3 staining in mouse hippocampal CA3 region appeared in the pyramidal cell body (P and arrows).
    References
    1. Gong, J. et al. (2006) Brain Res. 1082, 73.
    2. Fremeau, R.T. et al. (2004) Science 304, 1815.
    3. Schafer, M.K. et al. (2002) J. Biol. Chem. 277, 50734.
    4. Liguz-Lecznar, M. and Skangiel-Kramska, J. (2007) Acta Neurobiol. Exp. 67, 207.
    5. Akil, O. et al. (2012) Neuron 75, 283.
    6. Seal, R.P. et al. (2009) Nature 462, 651.
    Scientific background

    Central nervous system neurons have traditionally been thought to express exclusively membrane transporters and/or vesicular transporters for their transmitter. Three vesicular glutamate transporters (VGLUTs) have recently been cloned: BNPI/VGLUT1 (a brain-specific sodium dependent inorganic phosphate (Pi) transporter), and its homologs DNPI/VGLUT2 (differentiation-associated sodium-dependent Pi transporter) and VGLUT31. These transporters mediate glutamate uptake inside presynaptic vesicles and are anatomical and functional markers of glutamatergic excitatory transmission2.

    VGLUT 1-3 are very similar in structure and function, but are used by different neuronal populations. VGLUT3 is a glycosylated transmembrane protein with ten putative transmembrane domains (TMD I–X) and both amino- and carboxyl-termini located in the cytosol3.

    VGLUT3 is localized in a limited number of glutamatergic neurons in multiple brain regions: neocortex, hippocampus, olfactory bulb, hypothalamus, substania nigra and raphe nuclei. Additionally, VGLUT3 is expressed in a population of symmetrical synapses. It is detected in hippocampal and cortical GABAergic neurons, cholinergic neurons in the striatum and serotonergic neurons in the raphe nuclei4.

    Mice lacking VGLUT3 are congenitally deaf due to loss of glutamate release at the inner hair cell afferent synapse5. In addition loss of VGLUT3 specifically impairs mechanical pain sensation6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Vesicular glutamate transporter 3. Anti-VGLUT3 Antibody (#AGC-037) can be used in western blot and immunohistochemistry applications. It has been designed to recognize VGLUT3 from rat, mouse and human samples.

    For research purposes only, not for human use
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