Anti-VMAT2 Antibody

Vesicular monoamine transporter 2, SLC18A2, SVAT, VAT2
    Cat #: AMT-006
    Alternative Name Vesicular monoamine transporter 2, SLC18A2, SVAT, VAT2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)KHEKNSTEIQT(A)R, corresponding to amino acid residues 52-64 of rat VMAT2 (Accession Q01827). 1st luminal loop.
    • Anti-VMAT2 Antibody
    Accession (Uniprot) Number Q01827
    Gene ID 25549
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse, human – 12/13 amino acid residues identical.
    RRID AB_2340996.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: if, ih, wb
    May also work in: ic*, ifc*, ip*
    Western blot
    • Anti-VMAT2 Antibody
      Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat brain stem (lanes 3 and 6) lysates:
      1-3. Anti-VMAT2 Antibody (#AMT-006), (1:400).
      4-6. Anti-VMAT2 Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-VMAT2 Antibody
      Expression of VMAT2 in rat substantia nigra dopaminergic neurons
      Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-VMAT2 Antibody (#AMT-006), (1:1000). A. VMAT2 staining (red). B. Tyrosine hydroxylase staining (green), a marker of dopaminergic neurons. C. Merger of A and B demonstrates VMAT2 expression in several dopaminergic neurons (arrows). Cell nuclei were stained with DAPI (blue).
    References
    1. Bernstein, A.I. et al. (2014) Neurochem. Int. 73, 89.
    2. Weihe, E. et al. (1994) J. Mol. Neurosci. 5,149.
    3. Sudhof, T.C. (2004) Annu. Rev. Neurosci. 27, 509.
    4. Fon, E.A. et al. (1997) Neuron 19, 1271.
    5. Zheng, G. et al. (2006) AAPS J. 8, E682.
    Scientific background

    Vesicular monoamine transporters (VMATs) are essential for proper monoaminergic neurotransmission, which requires the sequestration of the transmitter into synaptic vesicles by VMAT for subsequent Ca2+-stimulated exocytotic release. VMATs contain 12 transmembrane spanning domains, with cytosolic C- and N-terminals and large glycosylated intravesicular loops. In mammals, there are two VMAT isoforms: VMAT1 (SLC18A1) and VMAT2 (SLC18A2)1.

    VMATs primarily transport monoamines (dopamine, serotonin, norepinephrine and histamine), but also sequester toxicants into vesicles, shunting them away from cytosolic sites of action. In the central nervous system, VMAT2 is the only transporter that moves cytoplasmic dopamine (DA) into synaptic vesicles for storage and subsequent exocytotic release2.

    VMAT2 is expressed in monoaminergic neurons of the central nervous system (CNS) and sympathetic postganglionic neurons. VMAT2 is widely expressed during embryonic development3.

    Homozygous knock-out of VMAT2 in mice is lethal and heterozygous knock-out of VMAT2 in mice shows a clear gene dosage effect, with half the amount of dopamine, norepinephrine, and serotonin content in brain4. In addition VMAT2 plays a role in neuroprotection and molecules that interact with VMAT2 may have value as treatments for diseases such as Parkinson’s disease5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 30/01/2020

    Anti-VMAT2 Antibody (#AMT-006) is a highly specific antibody directed against an epitope of the rat vesicular monoamine transporter 2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize VMAT2 from human, rat and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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